摘要
利用过碘酸钠法用辣根过氧化物酶标记了水稻条纹病毒(RSV)的3株单克隆抗体,3株酶标抗体(HRP-3B9、HRP-2H2、HRP-2E5)的效价分别达到1:25600、1:1600、1:3200,直接ELISA方阵试验确定HRP-3B9的最佳工作浓度分别为1:5000.用HRP-3B9在硝酸纤维素膜上采用直接和间接斑点免疫结合试验(DIBA)对灰飞虱体内RSV进行了检测.结果表明,DIBA法可有效地用于检测灰飞虱的带毒率,直接DIBA法比间接DIBA法灵敏度更高.故采用直接DIBA法对采自江苏10个县、市的灰飞虱带毒率进行了检测.
Three horseradish peroxidase-linked monoclonal antibodies of Rice stripe virus (RSV), HRP-3B9, HRP-2H2 and HRP-2E5, were produced by NaIO_4 method. The diluted times of the three enzyme-linked antibodies were 1∶25600, 1∶1600 and 1∶3200, respectively. In direct ELISA, the suitable diluted times of HRP-3B9 was 1∶5000. Enzyme-linked antibody HRP-3B9 was selected to detect RSV in Laodelphax striatellus Fallen with direct dot immunobinding assay (DIBA) and indirect DIBA, and the detection results showed that the direct DIBA was more sensitive than indirect DIBA. The direct DIBA was then used to detect the virus in Laodelphax striatellus Fallen collected from 10 counties in Jiangsu province, China.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2005年第1期37-40,共4页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省农业成果转化资金(G20030724)
江苏省重点攻关项目(BE2001339)
江苏省重大基础研究计划(BK2003002)
江苏省政府专项资助.
关键词
水稻条纹病毒
DIBA
酶标抗体
灰飞虱
Rice stripe virus
DIBA
Enzyme-linked antibodies
Laodelphax striatellus
