节杆菌(Arthrobacter)42-1菌株的分离、鉴定及产肌氨酸氧化酶条件研究

Isolation and characterization of an Arthrobacter strain 42-1 and study on the conditions for its sarcosine oxidase production.

从42℃的恒化富集培养物中分离到一株产肌氨酸氧化酶(SOX)的节杆菌.对菌株42-1产酶发酵条件的研究表明,SOX可以被诱导物如肌氨酸、肌酸、肌酐和氯化胆碱诱导产生.在发酵过程中适当减少通气量对SOX的产生有显著的促进作用.葡萄糖等容易利用碳源的存在对SOX合成不产生降解代谢产物的抑制作用.而尿素的存在则对SOX生成有强抑制作用.因而SOX分解肌氨酸的主要作用是为细胞提供生长所需的氮源.

A sarcosine oxidase (SOX)-producing Athrobacter 42-1 was isolated from a chemostat enrichment of 42 ℃. The results showed that SOX from strain 42-1 was an induced enzyme and could be induced by reagents, such as sarcosine, creatine, creatinine and choline chloride. When aeration was suitably reduced, the activity of SOX would be remarkably increased. The easily used carbon sources, such as glucose, had no catabolic repression effect on SOX production, while urea could dramatically repressed the production of SOX. Therefore, the primary function of creatine decomposition was to provide nitrogen source for the cell growth.