亚砷酸对人肝癌细胞生长增殖及分化的影响

The influence of arsenious acid on proliferation and differentiation of human hepatocarcinoma cells

目的探讨亚砷酸对人肝癌细胞BEL-7402增殖、分化的作用.方法通过体外细胞培养,用四氮唑盐(MTT)比色法观察细胞生长曲线的变化,台盼蓝拒染法观察细胞群体倍增时间(TD)的改变,并分别用自动化学发光法、生化法检测亚砷酸对细胞甲胎蛋白(AFP)合成及谷氨酰转肽酶(GGT)、乳酸脱氢酶(LDH)活性的影响,用苏木精-伊红(HE)染色法及透射电镜观察细胞的分化形态特点.结果亚砷酸(1.0～8.0 μmol/L)能抑制BEL-7402细胞生长增殖,呈剂量、时间依赖性.亚砷酸(8.0 μmol/L)对BEL-7402细胞AFP合成及GGT、LDH活性有显著的抑制作用,HE染色及透射电镜观察可见部分细胞呈现分化的形态改变.结论亚砷酸体外能够抑制人肝癌细胞生长增殖,并具有一定的诱导分化作用.

Objective To investigate the effect of arsenious acid on proliferation and differentiation by HCC cell line BEL 7402 Methods The cell culture and MTT assay were used to study the change of cell growth curve, and the method of trypan blue exclusion was used to observe the alteration of TD. The production of AFP and the specific activities of GGT, LDH were measured by automated chemiiluminescence system and biochemical method respectively The morphologic changes of cell differentiation were observed by HE dying and transmission electron microscope. Results Arsenious acid in concentrations from 1 0 μmol/L to 8 0 μmol/L inhibited the growth and proliferation of BEL 7402 cells in a dose and time dependent manner.After treatment with 8 0 μmol/L,the amount of AFP production and the specific activities of GGT、LDH were significantly decreased, morphologic changes of cell differentiation occurred in some of the cells. Conclusion In vitro , arsenious acid could inhibit the growth and proliferation of human hepatocarcinoma cells, and it also could induce cell differentiation.