食管鳞状细胞癌Fhit蛋白表达与吸烟的关系

The expression of Fhit protein in esophageal squamous cancer and its relation to smoking

目的 探讨食管鳞状细胞癌Fhit蛋白表达与吸烟的关系。方法 采用免疫组化SP法检测 83例食管鳞状细胞癌Fhit蛋白表达并分析其与吸烟、组织学分级、临床分期以及淋巴结转移的关系。结果 Fhit蛋白在食管鳞状细胞癌中表达减弱或缺失率为 60 2% (50 /83),吸烟组中Fhit蛋白表达减弱或缺失率为 72 0% ( 36 /50 )较非吸烟组 ( 42 4%,14 /33)高(P=0 007)。Fhit蛋白表达减弱或缺失在高分化鳞状细胞癌、中分化鳞状细胞癌、低分化鳞状细胞癌中分别为 60 9% ( 14 /23 )、62 8% ( 27 /43 )、52 9% ( 9 /17 ),差异无显著性(P=0 779);在临床分期中的分布Ⅰ期癌 33 3% (2 /6),Ⅱ期癌 57 9% (33 /57),Ⅲ期癌 75 0% (15 /20),差异无显著性(P=0 060),亦与淋巴结转移无关 (P=0 258 )。结论 食管鳞状细胞癌Fhit蛋白表达状况与吸烟有关。

Objective To explore the association between the expression of fragile histidine triad( Fhit) protein in esophageal squamous cancer and cigarette smoking. Methods We detected Fhit protein expression in 83 cases of esophageal carcinoma and analysed its relationship to cigarette smoking, histological grade,clinical stage and lymph nodes metastasis by immunohistochemical method. Results The loss of Fhit protein expression in esophageal Squamous cell cancer was 60 2%. The loss of expression Fhit protein in the case group was higher in the smokers compared to non smokers (72 0% vs 42 6%). The difference was significant ( P = 0 007). The loss of Fhit protein expression in grade of well differentiation, moderate differentiation and poor differentiation was 60 9%(14/23), 62 8%(27/43) and 52 9%(9/17), respectively and no difference was found between the grades of tumor( P= 0 779). No difference in the loss of expression Fhit protein was found between cancer at clinical stage(2/6,33 3%),Ⅱ(33/57,57 9%)and Ⅲ(15/20,75 0%),respectively( P= 0 060). The loss of Fhit protein expression in esophageal squamous cell cancer was not related to lymph nodes metastasis ( P= 0 258).Conclusion Fhit protein expression in esophageal squamous cell cancer is related to cigarette smoking.