Ephrin-B2在人胶质瘤中的表达及意义

Expression of Ephrin-B2 Gene in Human Glioma and Its Implications

目的探讨Ephrin-B2基因在人胶质瘤中的表达规律及意义。方法分别用免疫组织化学和Western印迹法检测4例正常脑组织和21例人脑胶质瘤中Ephrin-B2基因的表达。结果Western印迹法检测显示,人正常脑组织中未见Ephrin-B2基因明显表达不同病理级别胶质瘤组织均有不同程度,Ephrin-B2蛋白表达,Ⅲ级、Ⅳ级表达较强,分别与Ⅰ、Ⅱ级比较,差异均有显著性(P<0.01),Ⅲ级与Ⅳ级之间以及Ⅰ与Ⅱ级之间比较,差异无显著性(P>0.05)。免疫组化检测显示,Ephrin-B2蛋白除在肿瘤细胞中有表达外在各级别胶质瘤中血管内皮细胞表达呈强阳性结论。Ephrin-B2在胶质瘤发生、发展和血管生成中可能有重要作用。

Objective To investigate the expression of ephrin-B2 in human brain gliomas and its implication. Methods The expression of ephrin-B2 in 4 cases of the normal brain tissues and 21 cases of the gliomas were detected by immunohistochemical and Western blot methods. Results The expression of ephrin-B2 was not detected in the normal human brain tissues by Western blot method. The expression level of ephrin-B2 was significant higher in the gliomas of grade Ⅲ and Ⅳ respectively than that in the gliomas of grade Ⅰand Ⅱ (P<0.01), but there was insignificant difference in the ephrin-B2 expression between grade Ⅲ and grade Ⅳ or between grade Ⅰ and grade Ⅱ (P>0.05). The immunohistochemical method showed that the ephrin-B2 expression was not only found in the tumor cells, but also in the gliomas blood vessles endothelial cells which were stained deeper compared to the cytoplasm of the gliomas. Conclusions Ephrin-B2 may play an important role in the development and vasculogenesis of the glioma.