摘要
目的:研究三氧化二砷(As2O3)注射液、5 氟脲嘧啶(5-Fu)注射液单用和两药联合使用对人结肠癌裸鼠移植 瘤端粒酶活性及其催化亚单位表达的影响。方法:建立人类结肠癌裸鼠移植瘤模型,随机分为4组:生理盐水组;As2O3组 (2.5mg/kg);As2O3(2.5mg/kg)+5-Fu组(20mg/kg);5-Fu组(20mg/kg)。采用腹腔注射给药。利用银染—端粒重复扩增 (TRAP)法测定人结肠癌裸鼠移植瘤端粒酶活性、RT-PCR法检测端粒酶催化亚基(humantelomerasereversetranscriptases, hTERT)的表达。结果:生理盐水组和5 Fu组的端粒酶活性及其催化亚单位表达不受影响,As2O3单药组和联合用药组均明 显抑制端粒酶活性及其催化亚单位表达。结论:As2O3单用以及联合5 -Fu对于人结肠癌裸鼠移植瘤细胞的端粒酶活性及其 催化亚单位的表达均具有明显的抑制作用。
Objective:To study the influence of As 2O 3 or/and 5-Fu on hTERT expression and telomerase activity of human colonic carcinoma xenograft in nude mice.Methods:To establish human colonic carcinoma xenograft model in nude mice.As 2O 3 was administrated by i.p injection.We detected the telomerase activity by silver stain with TRAP.The expression of hTERT was detected by reverse transcription polymerase chain reaction(RT-PCR).Results:The tolemerase activity and hTERT expression of NS control group and 5-Fu group were highly expressed while those of As 2O 3 group and combination group were obviously inhibited.Conclusion:As 2O 3 injection singularly and in combination with 5-Fu can obviously inhibit tolemerase activity and hTERT expression of human colonic carcinoma xenograft in nude mice.
出处
《临床肿瘤学杂志》
CAS
2005年第1期11-13,共3页
Chinese Clinical Oncology
基金
南京市医学科技发展专项基金项目(YKK02477)
