抑癌基因p73 FHIT和PTEN蛋白表达与肺癌临床病理学特征及预后关系探讨

p73、FHIT and PTEN Protein Expression in Lung Cancers as Related to Clinico-Pathological Characteristics and Prognosis

目的:探讨抑癌基因p73、FHIT和PTEN与肺癌发生、发展的关系。方法:应用免疫组化SABC法检测65例肺癌组织、癌旁组织和正常肺组织中p73、FHIT和PTEN基因蛋白的表达。结果:肺癌组织中p73蛋白阳性表达率明显高于正常肺组织和癌旁组织(P<0.05),正常肺组织和癌旁组织中FHIT和PTEN蛋白阳性表达率明显高于肺癌组织(P<0.05);不同类型和不同分化程度肺癌组织中p73、FHIT和PTEN蛋白的阳性表达率比较差异无显著性(P>0.05);肺癌组织中p73、FHIT和PTEN蛋白阳性表达率与临床分期和患者预后有明显相关性(P<0.05)。结论:p73、FHIT和PTEN基因蛋白表达缺失在不同类型和不同分化程度在肺癌发生、发展中可能存在着普遍意义,三者可作为判断肺癌生物学行为和患者预后的参考指标。

Objective: To investigate the relationship between the expression of p73, FHIT and PTEN protein and the significance of p73, FHIT and PTEN gene in lung cancer. Methods: The expression of p73, FHIT and PTEN protein in 65 cases of lung cancer, adjacent tissues of cancer and normal tissues was tested by immunohistochemical SABC method. Results: The rate of expression of p73 protein in tumor tissues was significantly higher than that in adjacent tissues of cancer and normal tissues(P<0.05), but the rate of expression of FHIT and PTEN protein in tumor tissues was significantly lower than that in the adjacent tissues of cancer and normal tissues (P<0.05). It was shown that p73, FHIT and PTEN protein expression did significantly not correlate with the type and grade of the tumor(P>0.05),but it correlated with the clinical stage and survival time of the patients (P<0.05). Conclusions: The results suggest that p73 gene should not play main role of action in restraining lung carcinoma. but FHIT and PTET gene should play the main role of action in restraining lung carcinoma. The expression of p73? FHIT and PTEN protein should be used as an useful marker of the biological behavior of lung cancer and as an independent prognosis factor for the patients with lung cancer.