肝组织中Chymase浓度在自身免疫性肝病肝纤维化中的意义

Increased chymase in livers with autoimmune disease:Co-localization with fibrosis

探讨肝组织中Chymase(糜酶 )浓度在自身免疫性肝炎 (AIH)和原发性胆汁性肝硬化 (PBC)肝纤维化中的意义。采用酶联免疫吸附试验 (ELISA)检测 15例AIH、14例PBC患者肝组织中Chymase浓度 ,与 11例急性肝炎 (AH)患者作对比 ;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。结果AIH及PBC患者肝组织中Chymase浓度分别为 (15 5 3± 4 89)ng/mg和 (12 6 7± 3 6 9)ng/mg ,明显高于急性肝炎患者(2 72± 1 0 2 ) ,P <0 0 1;免疫组化染色结果 ,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁 ,其分布与纤维化部位相一致 ,且肝组织中Chymase浓度高的患者 ,其肝组织内Chymase标记的肥大细胞分布增多 。

To investigate spatial relatioship betwwen human chymase and fibrosis in liver specimens affected by autoimmune hepatitis(AIH)or primary biliary cirrhosis(PBC).The chymase levels were determined by using the method of enzyme linked immunosorbant assay(ELISA)in 15 cases of AIH and 14 cases of PBC.11 cases of acute hepatitis (AH) were used as control. Mast cell distribution in the specimens was determined immunohistochemically using anti-chymase antibody. The mean amounts of chymase in livers with AIH and PBC were (15.53±4.89)ng/mg and (12.67±3.69)ng/mg respectivly.Hepatic chymase in AIH and PBC was significantly more abundant than in AH (2.72±1.02)ng/mg,P<0.01.When sections from patients with AIH and PBC were immunostained for chymase,immunoreactive mast cells were detected in portal areas and sinusoidal walls,coinciding with zones of fibrosis. Chymase appears to be involved in hepatic fibrosis in AIH and PBC.