烟草花粉萌发和花粉管生长期间柱头和花柱中的钙分布

The Distribution of Calcium in the Stigma and Style of Tobacco During Pollen Germination and Tube Growth

烟草柱头表面有两层覆盖物,其中含有少量细 小的钙颗粒。花粉落到柱头上后,储存在花粉外壁中 的钙被释放到覆盖层中。当花粉管穿过覆盖层长入柱 头细胞之间时,花粉管顶端的细胞壁中出现了大量的 细小钙颗粒。开花后22 h观察时,在花柱引导组织中 形成了钙的梯度分布:花柱上部引导组织中的钙较少, 而下部连接子房处的花柱引导组织中含有较多的钙颗 粒。去雄花开花后1d时,花柱上部引导组织中的钙明 显增多;3d时,连柱头细胞中也出现了较多的钙颗粒。 讨论了烟草花柱引导组织中钙梯度分布和花粉管生长 的关系。

After pollen grains of tobacco landed on stigma they begin to hydrate and form many small vesicles containing some calcium grains in cytoplasm. The calcium stored in pollen wall is released into tectum of stigma to make a calcium-rich environment. When a pollen tube penetrates the tectum and grows between stigma cells, numerous calcium precipitates appear in the tip tube wall. The length of style of tobacco is 4 cm, and the pollen tube need take 44 h to reach the ovary. The style was artificially divided into 4 stages and each 1 cm respectively. There were only a few of calcium precipitates in the transmitting tissue of style from anthesis to 11 h after pollination (Plate Ⅱ-1). A calcium gradient in the transmitting tissue of style was formed at 22 h after pollination: only a few calcium precipitates found in the transmitting tissue of the style under stigma and at stage 1, 2 and 3 (Plate Ⅱ-3), and many of them were located in the transmitting tissue of style near ovary (stage 4) (Plate Ⅱ-4). When the flowers were emasculated and unpollinated at 1 d after anthesis, no calcium gradient in the transmitting tissue of style could be identified because some precipitates were also accumulated in the transmitting tissue at stage 1 (Plate Ⅲ-1). When a flower without pollination was kept for 3 d, some calcium precipitates were formed in the cells of stigma (Plate Ⅲ-4), and the cells of the whole transmitting tissue contained the same quantity of calcium precipitates (Plate Ⅲ-5,6). To check the ability of pollen to germi- nate and grow in a low calcium environment, pollen grains were cultured in a medium containing 0-0.1 % CaCl2·2H2O. The result of in vitro assay confirmed that tobacco pollen can germinate and the pollen tube can grow in an environment with a very low concentration of calcium, which may be similar to the environment in the stigma. A few calcium precipitates were accumulated in stigma and upper transmitting tissue of tobacco to make a calcium gradient in the style. If the calcium in the style at 1 cm increases it will be increased more at 4 cm, and more in ovules, and more in synergid cells to keep the calcium gradient. When the emasculated flowers were not pollinated for 3 days the calcium in upper transmitting tissue evidently increases. The calcium in style is abundant in all plants, but the distribution of calcium in style is different between different plant species. For this difference, it may differ from types of style, and in the plants with short style the calcium gradient in the style is too small to be detected. But for tobacco with style 4 cm long, the gradient can be identified using antimonate method.