除虫菊细胞的悬浮培养

Cell Suspension Culture of Pyrethrum cinerariifolium

除虫菊悬浮细胞从培养后第8天开始迅速生长,14d达到最大生长量,其最适培养基为:MS+4mg·L-12,4-D+0.1mg·L-1NAA+0.3mg·L-16-BA+30g·L-1蔗糖,最适接种量为15g·L-1,摇瓶装液的体积分数为40%,初始培养的最适pH值为5.8,收获时pH下降为4.6±1,最适培养温度为25℃。

The rapid growth of Pyrethrum cinerariifolium suspension culture cell was on the 8th day and the maximum growth amount was obtained on the 14th day. The optimum medium was MS added 2,4-D 4 mg·L-1 and NAA 0.1 mg·L-1, 6-BA 0.3 mg·L-1 and sucrose 30 g·L-1. The optimum culture situations were obtained as follows: seed volume was 15 g·L-1, the liquid medium volume per flask was 40%, the initial pH was 5.8, the last pH was 4.6±1, the optimum temperature was 25℃.