期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大鼠脑缺血再灌注损伤后死亡相关蛋白激酶的表达及意义

Expression and significance of DAPK after cerebral ischemia reperfusion in rats
下载PDF
导出
摘要 目的　探讨大鼠局灶性脑缺血再灌注损伤时死亡相关蛋白激酶 (Deathassociatedrelatedproteinkinase ,DAPK)的表达变化及其与脑缺血再灌注损伤的关系。方法　线拴法建立局灶性脑缺血再灌注模型 ,SD大鼠随机分为正常对照组、假手术组和缺血再灌注组 ,缺血组于栓塞 3h后分别再灌注 12h、2 4h、3d、7d。HE染色观察组织病理变化 ;免疫组化法检测DAPK的表达 ;原位杂交法检测DAPKmRNA的表达。结果　缺血组DAPK免疫阳性反应于再灌注后 2 4h开始增强并于 3d时达高峰 ,随后下降 ;DAPKmRNA的表达于再灌注 3d时达高峰 ,随后下降。结论　脑缺血再灌注损伤诱导DAPK表达增强 ; Objective To explore the alterations of death-associated related protein kinase (DAPK) expression and its relationship to cerebral ischemia reperfusion injury in rats.Methods The focal cerebral ischemia/reperfusion models of rats were achieved by middle cerebral artery occlusion (MCAO).The adult rats were randomly divided into normal control,sham-operation and ischemia/reperfusion groups.The ischemic group was respectively reperfused for 12h,24h,3d and 7d after ischemia for 3h.Pathological changes of injured neurons were demonstrated by H-E st aining,the expression of DAPK was assayed by immunohistochemistry and the expression of DAPK mRNA was detected by in situ hybridization.Results The expression of DAPK was strongly increased at 24h and peaked at 3d after reperfusion,then decreased.The peak expression of DAPK mRNA occurred at 3d after reperfusion.Conclusion These observations support that the expression of DAPK increases significantly in the cerebral tissues after reperfusion of focal cerebral ischemia and strongly supports a key role for DAPK in ischemia/reperfusion brain injury.
作者 吴旖琦 赵瑞波 吴鹤 田琳
机构地区 哈尔滨医科大学病理学教研室
出处 《哈尔滨医科大学学报》 CAS 北大核心 2005年第1期32-34,共3页 Journal of Harbin Medical University
关键词 脑缺血 DAPK 免疫组化 大鼠 cerebral ischemia DAPK immunohistochemistry rat
分类号 R-332 [医药卫生] R363 [医药卫生—病理学]
  • 相关文献

参考文献11

  • 1张海涛,朱振宇,吉琼梅,李秀英,李民友,马涧泉,梁念慈.DAPK1基因开放读码序列的克隆、序列分析及诱导Raji细胞凋亡[J].中国病理生理杂志,2004,20(1):88-93. 被引量：9
  • 2Pelled D,Raveh T,Riebeling C, et al.Death-associated protein(DAP) kinase plays a central role in ceramide-induced apoptosis in cultured hippocampal neurons[J] .J Biol Chem,2002,277(3):1957-1961.
  • 3Yamamoto M, Hioki T, Ishii T, et al. DAP kinase activity is critical for C(2)-ceramide-induced apoptosis in PC12 cells[J]. Eur J Biochem,2002,269( 1 ): 139-147.
  • 4Cohen O, Kimchi A. DAP kinase: from functional gene cloning to establishment of its role in apoptosis and cancer [ J ]. Cell Death Differ,2001,8(1) :6-15.
  • 5Cohen P. Protein kinase-the majoy drug targets of the twenty-first century[J]? Nat Rev Drug Discov,2002,1(4) :309-315.
  • 6Scapin G. Structural biology in drug design: selective protein kinase inhibitors [ J]. Drug Discov Today,2002,7(11) :601-611.
  • 7Nakatsuka S,Takakuwa T, Aozasa K. Hypermethylation of DAP kinase gene CpG island in malignant lymphoma with B cell phenotype[J] .Rin Sho Byori,2001,49(12): 1242-1247.
  • 8Cohen O, Inbal B, Kissil JL, et al. DAP- kinase participates in TNF-alpha and Fas-induced apoptosis and its function requires the death domain[J]. J Cell Biol, 1999,146( 1 ): 141-148.
  • 9Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989,20( 1 ):84-91.
  • 10Raveh T, Kimchi A. DAP kinase a proapoptotic gene that functions as a tumor suppressor[J]. Exp Cell Res,2001,264( 1 ): 185-192.

二级参考文献13

  • 1[1]Deiss LP,Feinstein E, Berissi H,et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death[J]. Genes Dev,1995,9(1):15-30.
  • 2[2]Cohen O, Inbal B, Kissil JL, et al. DAP-kinase participates in TNF-alpha- and Fas-induced apoptosis and its function requires the death domain[J]. J Cell Biol,1999,146(1):141-148.
  • 3[3]Inbal B, Cohen O, Polak-Charcon S, et al. DAP kinase links the control of apoptosis to metastasis[J]. Nature,1997,390(6656):180-184.
  • 4[4]Dori Pelled, Tal Raveh, Christian Riebeling, et al. Death-associated protein (DAP) kinase plays a central role in ceramide-induced apoptosis in cultured hippocampal neurons[J]. J Biol Chem,2002,277(3):1957-1961.
  • 5[5]Yamamoto M, Hioki T, Ishii T, et al. DAP kinase activity is critical for C(2)-ceramide-induced apoptosis in PC12 cells[J]. Eur J Biochem, 2002, 269(1):139-147.
  • 6[6]Simpson DJ, Clayton RN, Farrell WE. Preferential loss of death associated protein kinase expression in invasive pituitary tumours is associated with either CpG island methylation or homozygous deletion[J]. Oncogene,2002,21(8):1217-1224.
  • 7[7]Feinstein E, Druck T, Kastury K, et al. Assignment of DAP1 and DAPK-genes that positively mediate programmed cell death triggered by IFN-gamma-to chromosome regions 5p12.2 and 9q34.1, respectively[J]. Genomics,1995,29(1):305-307.
  • 8[8]Knowles MA, Elder PA, Williamson M, et al. Allelotype of human bladder cancer[J]. Cancer Res,1994,54(2):531-538.
  • 9[9]Kissil JL, Feinstein E, Cohen O, et al. DAP-kinase loss of expression in various carcinoma and B-cell lymphoma cell lines: possible implications for role as tumor suppressor gene[J]. Oncogene,1997,15(4):403-407.
  • 10[10]Raveh T, Kimchi A. DAP kinase-a proapoptotic gene that functions as a tumor suppressor[J]. Exp Cell Res,2001,264(1):185-192.

共引文献8

哈尔滨医科大学学报
2005年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部