人参皂甙Rg3对肺腺癌A549细胞株信号传导相关基因表达的影响

The effect of 20(R)-ginsenoside Rg3 on the differential expression of cell signaling genes and other related genes in human lung adenocarcinoma cell line A549

目的 探讨人参皂甙Rg3[2 0 (R) Rg3]对人肺腺癌A5 4 9细胞株的信号传导相关基因差异表达的影响。方法 A5 4 9细胞株分 2组进行培养 ,实验组培养液中含有 10 -6mol/L的2 0 (R) Rg3,对照组不加药物 ,干预培养 72h后用BiostarH 4 0s型基因芯片测定其基因差异表达。结果 共检测到有效基因点数量 3490个 ,2 0 (R) Rg3干预后有 2 4个基因发生差异表达 ,其中 2个细胞信号和传递蛋白基因以及 2个细胞骨架和运动基因上调 ,原癌基因和抑癌基因下调的有 3个 ,蛋白翻译合成的基因 2个下调、1个上调 ,DNA合成修复重组和代谢相关基因 1个、免疫相关基因 1个下调 ,代谢基因 1个上调 ,其他功能基因也受到一定影响。结论 2 0 (R) Rg3可显著影响人肺腺癌A5 4 9细胞株信号传导等相关基因的差异表达。

Objective To study the effect of 20(R)-ginsenoside Rg3 [20(R)-Rg3] on the differential expression of cell signaling genes and other related genes in human lung adenocarcinoma cell line A549. Methods The cell line A549 was cultured with 20(R)-Rg3 (10 -6mol/L) for 72 h, RNA was extracted, and the differential expression of cell signaling genes and other related genes were detected with DNA microarray. Results A total of 3 490 genes were detected, and the expression of 24 genes were changed after the addition of 20(R)-Rg3. Two cell signaling genes & transducin genes were up-regulated. Two cystoskeleton & locomotion genes were up-regulated. Three proto-oncogene & anti-oncogene genes were down-regulated. Two of the translation & synthetical genes were down-regulated, and 1 of them was up-regulated. One DNA recombination gene and one metabolism gene were down-regulated. Conclusion 20(R)-Rg3 showed significant effect on the differential expression of cell signaling genes and other related genes in human lung cell line A549.