活化因子Ⅶ及其基因MspⅠ多态性与高血压合并冠心病的关系

The relationship between activated coagulation factor Ⅶ,its gene MspI polymorphism,and coronary artery disease with hypertension

目的:探讨血浆活化因子Ⅶ(FⅦa)及其基因MspI多态性与中国汉族人高血压合并冠心病的关系。方法:采用候选基因与病例对照的方法,以聚合酶链反应及限制性内切酶片段长度多态性(PCR-RFLP)分析技术行FⅦ基因MspI多态性分析,同时采用重组可溶性组织因子法(rSTF2-219)测定血浆FⅦa水平。结果:①冠心病组血浆FⅦa水平显著高于高血压组与正常对照组,FⅦa与高血压合并冠心病的危险性独立相关;②FⅦ基因型及等位基因频率分布在各组间差异均无统计学意义;③各组血浆FⅦa水平均与FⅦ基因多态性显著相关。结论:血浆FⅦa水平增高是高血压合并冠心病的独立危险因素,FⅦa水平受其基因MspI多态性的影响。

Objective: To investigate the association of activated coagulation factor Ⅶ (FⅦa) and its gene MspI polymorphism with coronary heart disease combined hypertension of Han nation in Chinese. Methods: This study was performed with the method of candidate gene and case-control study. FⅦ genotypes were identified with polymerase chain reaction amplified genomic deoxyribonulieic acid (DNA) and MspI restriction fragment length polymorphism analysis,and plasma FⅦa levels were detected with recombinant tissue factor method. Results: ①Plasma FⅦa levels were significantly higher in patients with coronary heart disease than those in patients with hypertension. FⅦa was a risk factor for coronary heart disease with hypertension. ②The results suggested that the distribution of genotype and allelic frequencies had no significant difference in each group. ③FⅦa levels were associated with FⅦ gene polymorphism. Conclusion: Plasma FⅦa level may be an independent risk factor of coronary heart disease with hypertension and the level is influenced by MspI polymorphism of the FⅦ gene.