β-七叶皂甙钠对大鼠脑出血后血SOD、NO、TNF-α的影响

Effect of β-aescinate on changes of superoxide dismutase,nitrogen monoxide and tumor necrosis factor-α content in blood after intracerebral hemorrhage in rats

目的:观察β-七叶皂甙钠对大鼠脑出血后血中超氧化物歧化酶(SOD)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)的影响,探讨其治疗作用的机制。方法:将实验大鼠随机分为假手术1、2组;模型1、2组;七叶皂甙1、2组,采用胶原酶诱导大鼠形成脑出血模型,术后2h七叶皂甙1、2组腹腔注射β-七叶皂甙钠(2.8mg/kg),另4组注射生理盐水,分别在3、7d后取血,测定血SOD、NO、TNF-α含量。结果:与假手术组比较,模型组血SOD、NO、TNF含量明显下降和升高(P<0.01),与模型组比较,七叶皂甙组血SOD、NO、TNF含量明显升高和下降(P<0.01)。结论:β-七叶皂甙钠对脑出血后神经细胞损伤具有保护作用,其机制可能与抗自由基抑制NO和TNF毒性作用有关。

Objective: To observe the effect of β-aescinate on changes of superoxide dismutase(SOD),nitrogen monoxide(NO) and tumor necrosis factor-α(TNF-α) content in blood after intracerebral hemorrhage in rats and to explore its mechanism on treatment. Methods: Rats were randomly divided into six groups: sham-operative group1 and 2,model group1 and 2,aescinate group1 and 2. Collagenase was injected into caudate nucleus to establish rat model of intracerebral hemorrhage. After 2 hours,β-aescinate was injected by abdomen with a dose of 2.8mg/kg in aescinate group1 and 2,saline was injected in the other 4 groups. These rats were killed 3,7 days later. The content of SOD,NO,TNF was measured in blood. Results: Compared with sham-operative group,SOD content and NO,TNF-α content in blood were obviously lower and higher(P<0.01) in model group. Compared with model group,SOD content and NO,TNF-α content in blood were obviously higher and lower(P<0.01) in aescinate group. Conclusion: β-aescinate has an evident protective effect on injured neuron after intracerebral hemorrhage in rats,which may be related to the increasing of SOD content and the decreasing of NO,TNF-α content in blood.