荧光定量PCR与W-S银染检测幽门螺杆菌的对比研究

Comparison of fluorescence quantitative PCR assay and warthin-starry argentation for detection of helicobacter pylori

目的:建立一种简便、灵敏、准确而快速定量检测幽门螺杆菌(Hp)的方法,为临床诊断和药物疗效动态观察提供科学依据。方法:随机选择慢性胃病患者164例,在胃镜下取胃液2～3ml,应用荧光定量聚合酶链反应(FQ-PCR)扩增技术,对幽门螺杆菌16SrRNA基因进行定量检测;同时在胃镜下为164例患者取病变部位的粘膜组织,进行W-S银染色作为对照组。结果:FQ-PCR法和胃粘膜组织Warthin-Starry(W-S)银染检测Hp阳性率分别为47.56%(78/164)和40.85%(67/164),两者的符合率为91.5%。结论:FQ-PCR方法是一种简便、准确、无创伤性基因诊断Hp的方法,便于临床推广应用。

Objective: To provide scientific proof for diagnosis and dynamic observing the effect of medication by establishing a simple,sensitive,accurate and fast method of quantitative assay for helicobacter pylori. Method: One hundred and sisty-four patients with chronic gastropathy were random sampled,whose gastric juice was taken 2-3ml under the gastroscope. The 16S ribosomal RNA (16 S rRNA) gene of helicobacter pylori was quantitatively detected by fluorescence quantitative PCR (FQ-PCR) assay. The other 164 patients were taken gastric mucosa in the disease area and done warthin-starry argentation (W-S) as a control. Results: The positive rates of helicobacter pylori by FQ-PCR and W-S assay were 47.56% (78/164) and 40.85% (67/164) respectively. Coincident rate of both methods was 91.5%. Conclusion: FQ-PCR is a simple,accurate and noninvasive gene diagnostic method for helicobacter pylori infection,and it is easy to be popularized in clinical practice.