虫草复方制剂对动脉粥样硬化的作用机制

The therapeutic effect and its mechanism of chongcao compound on experimental atherosclerosis

目的:研究红细胞(RBC)免疫粘附功能与动脉粥样硬化的关系,观察虫草复方制剂治疗动脉粥样硬化的作用和机制。方法:雄性家兔20只,喂饲高脂饲料12周,然后喂饲加虫草复方制剂的高脂饲料8周,测定高脂饲料前后和药物治疗后的红细胞C3b受体花环形成率(RBC-C3bRR)、RBC免疫复合物花环形成率(RBC-ICR)、循环免疫复合物(CIC)和血脂水平;采用超声技术测量高脂饲料前后和药物治疗后兔腹主动脉最大内膜-中层厚度(IMTm)。结果:与实验前比较,喂饲高脂饲料后血脂明显升高;IMTm增厚犤(0.32±0.02)mmvs(0.89±0.19)mm,P<0.01犦;RBC-C3bRR降低犤(10.36±2.14)%vs(6.44±1.95)%,P<0.01犦;RBC-ICR升高犤(13.25±2.97)%vs(20.27±3.02)%,P<0.01犦;CIC升高犤156.60±40.27vs537.55±141.95,P<0.01犦;血脂水平和CIC均与IMTm正相关。与喂饲高脂饲料后比较,药物治疗后血脂水平下降,IMTm降低犤(0.89±0.19)mmvs(0.52±0.18)mm,P<0.001犦;RBC-C3bRR升高犤(6.44±1.95)%vs(9.77±1.25)%,P<0.001犦;RBC-ICR降低犤(20.27±3.02)%vs(15.21±1.92)%,P<0.001犦;CIC降低犤537.55±141.95vs233.45±36.05,P<0.001犦。结论:RBC免疫功能减低是动脉粥样硬化的重要危险因素之一,虫草复方制剂可能是一种有前途的抗动脉粥样硬化药物,值得深入研究。

Objective: To evaluate the therapeutic effect and its mechanism of chongcao compound on experimental atherosclerosis in rabbits induced by high-fatty diet. Methods: Twenty male rabbits were fed a high-fatty diet for 12 weeks,then were added chongcao compound for last 8 weeks. Serum lipid profile,rosette rate of red blood cell C3b (RBC-C3bRR),the rosette rate of red blood cell immune complexes (RBC-ICR),and circulating immune complexes (CIC) were measured before and after high-fatty diet and aftber chongcao compound respectively. The maxim intimal-medial thickness (IMTm) of abdominal aorta was measured by echocardiography at the same time. Results: Serum lipid level was elevated,RBC-ICR,CIC,IMTm increased [(13.25±2.97)% vs (20.27±3.02)%,P<0.01; (156.60±40.27)μ vs (537.55±141.95)μ,P<0.01; (0.32±0.02)mm vs (0.89±0.19)mm,P<0.01 respectively],and RBC-C3bRR decreased [(10.36±2.14)% vs (6.44±1.95)%,P<0.01)] significantly after 12 weeks high-fatty diet compared with base line. Linear correlation analysis demonstrated that serum lipid level and CIC has a relation with IMTm. After 8 weeks chongcao compound compared with 12 weeks high-fatty diet,serum lipid level,RBC-ICR,CIC,IMTm decreased [(20.27±3.02)% vs (15.21±1.92)%,P<0.001; (537.55±141.95)μ vs (233.45±36.05)μ,P<0.001; (0.89±0.19)mm vs (0.52±0.08)mm,P<0.001 respectively] and RBC-C3bRR increased [(6.44±1.95)% vs (9.77±1.25)%,P<0.001] significantly. Conclusion: The decreasing of red cell immune adhesive ability may be an important risk factor of the atherosclerosis. Chongcao compound can regress experimental atherosclerosis. The mechanism appears to be related to the decrease of serum lipid level and the improvement of the red cell immune adhesive capacity.