粤东产钩吻生物碱提取物的红外光谱与核磁共振谱分析

Infrared spectrum and nuclear magnetic resonance analysis of the alkaloid extract of Gelsemium produced in east Guangdong province

目的研究粤东产钩吻生物总碱提取物的结构.方法采用傅里叶变换红外光谱(FTIR)、核磁共振氢谱(1HNMR)与碳谱(13CNMR)、无畸变极化转移增益(distortionless enhancement by polarization transfer,DEPT)波谱分析及元素分析阐明该提取物的化学结构.结果在4000.0～400.0 cm-1区域内FTIR谱图显示各基团的特征吸收峰;样品的1HNMR与13CNMR指纹图均有高度的特征性和重现性;元素含量与理论值接近.结论提取物的结构分析与已知化合物钩吻素子结构相符.对粤东产钩吻总生物碱的结构鉴定将为将钩吻开发为安全有效的单体临床制剂、建立中药指纹图谱打下基础.

Objective To identify the structure of the alkaloid extract of Gelsemium from east Guangdong province of China. Methods Fourier transform infrared (FTIR) absorption spectrometry, hydrogen and carbon spectra of nuclear magnetic resonance (NMR) and distortionless enhancement by polarization transfer (DEPT) analysis were used for the structural identification of the alkaloid exstract of Gelsemium. Results The frequency, intensity and shape of the extract's characteristic peaks in infrared absorption spectra (4 000.0-400.0 cm-1) and 1H NMR, 13C NMR were recognized and compared. The molecular structure of the sample was consistent with the theoretically derived model. Conclusion The extract is structurally identical to koumine, which may provide evidence for its safe clinical application and establishment of Chinese medicine fingerprint database of Gelsemium.