小麦抗病种质贵农775中抗白粉病基因的RAPD标记

RAPD Markers Linked to the Powdery Mildew Resistance Gene in Wheat Line Gui-nong 775

运用RAPD技术 ,采用分离群体分组分析法 (BSA)进行了小麦种质贵农 775抗白粉病基因连锁的分子标记研究 ,其中有一个引物S2 0 18在抗病亲本贵农 775和抗病材料中扩增出了特异的DNA片段 ,而在感病材料和感病亲本丰产 3号中没有扩增出同样的DNA片段。此片段长度约为 880bp。用F2 分离群体 (10 6株植株 )进行遗传连锁性分析 ,引物S2 0 18880 扩增出的特异DNA片段与贵农 775抗白粉病基因紧密连锁 ,遗传距离为 8 9cM。此连锁标记还经其他相关后代材料的检测证实了其可靠性。

Random Amplified Polymorphic DNA (RAPD) was employed to detect molecular markers linked to powdery mildew resistance gene in wheat line Gui-nong 775. One DNA fragment was amplified in the resistant parent Gui-nong 775 and resistant materials by a primer S2018, but not in the susceptible materials and susceptible parent Feng-chan 3. The DNA fragment length was about 880 bp. The genetic linkage of the DNA fragment with powdery mildew resistance gene was tested on a segregating F 2 population (106 plants). The result showed that the DNA fragment was closely linked to the powdery mildew resistance gene in wheat line Gui-nong 775, in which the genetic distance between S2018 880. and powdery mildew resistance gene was 8.9 cM. The linkage molecular markers were approved to be credible by other relative progeny materials.