原位固化形成多孔碳酸化羟基磷灰石代骨材料的特征

Features of a bone substitute formed by in situ solidification of porous carbonated hydroxyapatite cement

目的:碳酸化羟基磷灰石骨水泥(carbonatedhydroxyapatitecement,CHC)能原位固化形成骨矿物相材料,是一种新型的无机代骨材料,但因缺乏自然骨的孔隙结构,降解、成骨速度非常缓慢。为此研究发泡成孔的方法,制备能原位固化形成多孔结构的CHC,以加快其降解速度,提高成骨能力。方法:实验于1997-01/2000-11由解放军总医院和中国科学院化学研究所共同完成。合成CHC,调整配方,添加成孔剂,制备能原位固化形成多孔结构的碳酸化羟基磷灰石代骨材料,并完成组织相容性、理化特性、力学强度以及内部结构等相关检测。结果:通过改变CHC的组分,添加成孔剂,可以成功的制备原位固化形成多孔结构的CHC。量化材料的组配成分,可调控其孔隙率和孔结构。系列检测结果表明,该材料固化产物为碳酸化羟基磷灰石,与人骨矿物相类似;材料的孔隙率为36%,孔之间互相贯通;固化时间为13～15min,能满足临床应用;抗压强度为(5.6±2.2)MPa,与骨松质强度相当;细胞毒性检测,组织相容性良好。结论:多孔CHC可塑形性强,适合填充任何不规则形状的骨缺损,可用于非负重部位的骨缺损修复。

AIM: Carbonated hydroxyapatite cement(CHC) can be solidified into bone mineral phase material, which is a new inorganic material of bone substitute, but it is very slow in degradation and osteogenesis for lack of the porous structure of natural bone. This paper aims to study the method of forming hole and establish porous CHC (PCHC) formed by in situ solidification, so as to accelerate its speed of degradation and improve its ability of osteogenesis.METHODS: The experiment was completed in cooperation of the General Hospital of Chinese PLA and the Institute of Chemistry of Chinese Academy of Sciences from January 1997 to November 2000. CHC was synthesized, formula was adjusted,porous agent was added, and the bone substitute was formed by in situ solidification of PCHC, and then the compatibility, physio chemical character, mechanical intensity and internal structure were determined.RESULTS: PCHC could be successfully established by in situ solidification through change of CHC component and addition of porous agent. The pore size and porosity character could be controlled and adjusted by quantifying the components of the material. The checking results demonstrated that the solidified product of this material was CHC, which was similar to the mineral phase of natural cancellous bone, the porosity was 36% with interconnect pore, the solidifying time was 13 to 15 minutes, which was satisfactory to the clinical application; The compressive strength was (5.6± 2.2) MPa, which was equal to the strength of cancellous bone. The cytotoxicity test showed an excellent histocompatibility.CONCLUSION: PCHC has a good shaping ability, and suits for filling bone defects of any irregular shapes,so it can be used to repair of bone defects at non loading parts.