异种完全脱蛋白骨复合自体红骨髓体内的异位成骨能力(英文)

Ectopic osteogenetic ability of heterogenous composite fully deproteinized bone compounded by autologous red marrow in vivo

背景:骨组织工程发展很快,但目前缺乏理想的支架材料,体外构建的组织工程化活性骨在体内的成骨能力各家报道不一。目的:探讨异种完全脱蛋白骨支架材料复合自体红骨髓体内植入的成骨作用。设计:随机对照的实验研究。单位:解放军济南军区第一五二医院骨科,平顶山市第一人民医院老干部病房,平顶山煤业集团朝川矿医院骨科。材料:实验在济南军区第一五二医院动物实验室完成。4月龄日本大耳白兔40只,体质量2.0～2.5kg,雌雄不限(平顶山市第一五二医院动物实验室提供)。干预:将经物理、化学方法处理制得的小牛完全脱蛋白骨(CFDB)支架材料与兔自体红骨髓复合后植入兔大腿肌肉内(40只)进行实验,术后4,8周分别进行检测。主要观察指标:①常规组织学检查。②碱性磷酸酶活性测定。③植入物成骨的定量判断。结果:实验组4周和8周移植物组织块ALP活性分别为(63.48±0.873)和(69.527±0.635)IU/L,移植物组织块成骨含量分析分别为(2.50±0.38)和(4.70±0.67)分,对照组4周和8周移植物组织块ALP活性分别为(2.50±0.38)和(4.70±0.67)IU/L,移植物组织块成骨含量分析分别为(1.90±0.54)和(3.40±0.54)分,表明,实验组在同时间点的成骨能力显著高于对照组,并随植入时间的延长,新骨形成增多。结论:异种完全脱蛋白骨支架材料可作为?

BACKGROUND:Although bone tissue engineering has been developed rapidly,ideal scaffold materials are deficient and the ability of tissue engineered bone constructed in vitro was reported inconsistently. OBJECTIVE:To study the ectopic osteogenesis of the implantation in vitro with composite fully deproteinized bone(CFDB) compounded by autologous red marrow. DESIGN: A randomized controlled study. SETTING:Department of Orthopaedics,the 152 Hospital of Jinan Military Area Command of Chinese PLA;Ward for Retired Cadres,First People's Hospital of Pingdingshan City;Department of Orthopaedics,Chaochuan Mine Hospital of Pingdingshan Coal Industrial Group. MATERIALS:The study was completed in the Department of Orthopaedics,the 152 Hospital of Jinan Militatry Area Command of Chinese PLA.Totally 40 Japanese flap eared white rabbits of 4 months old of either gender with a body mass from 2.0 kg to 2.5 kg were involved (provided by the Laboratory for Experimental Animals of the 152 Hospital in Pingdingshan city). INTERVENTION:Calf CFDB scaffold materials were compounded by rabbit autologous red marrow after physical and chemical managements,which were then implanted into the thigh muscles of 40 rabbits.The osteogenetic abilities of the materials compounded by autologous red marrow were analyzed at 4 weeks and 8 weeks after operations respectively. MAIN OUTCOME MEASURES:① Routine histological examination; ② alkaline phosphatase(ALP) activity assay;③ osteogenetic quantitative analysis of the implanted bone. RESULTS:ALP activities were(63.48± 0.873) and (69.527± 0.635) IU/L respectively,and the results of osteogenetic quantitative analysis were(2.50± 0.38) and(4.70± 0.67) points of rabbits in the study group at week 4 and week 8 respectively.ALP activities were(2.50± 0.38)and(4.70± 0.67) IU/L and the results of osteogenetic quantitative analysis were(1.90± 0.54 ) and(3.40± 0.54) points of rabbits in the control group at week 4 and week 8 respectively.The results indicated that the osteogenetic ability of the study group was significantly higher than that of the control group at the same time point, and the neogenetic bone increased along with the prolongation of the implantation time. CONCLUSION: CFDB could be applied as scaffold materials for bone tissue engineering,and its osteogenesis increases significantly after being compounded by autologous red marrow.