摘要
目的测定中药柴胡(Bupleurun Chinese DC，BCDC)提取物对人肝癌细胞BEL-7402细胞内VCR蓄积的影响，以探索柴胡逆转BEL-7402细胞多药耐药的机制．方法高效液相色谱法测定BCDC作用于BEL-7402后细胞内VCR蓄积情况的变化、结果柴胡可使人肝癌细胞BEL-7402细胞内VCR浓度升高(P<0．01)．结论柴胡可以增加VCR在BEL-7402细胞内的积聚浓度，部分逆转BEL-7402细胞的MDR．
ObjectiveTo measure the effects of the extracts from BCDC on cellular VCR concentration of human hepatoma BEL-7402 cells, in order to explore the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma.MethodsCellular VCR concentration was examined by high performance liquid chromatography(HPLC).ResultsThe BCDC can increase cellular VCR concentration of BEL-7402 cell.ConclusionBCDC could increase intracellular VCR concentration of BEL-7402 cell line.
出处
《北华大学学报（自然科学版）》
CAS
2005年第1期62-64,共3页
Journal of Beihua University（Natural Science）
基金
吉林省教育厅科研基金项目(吉教合字2000第26号)
关键词
柴胡
长春新碱
BEL-7402细胞
高效液相色谱法
Bupleurun Chinese DC
Vincristine
BEL-7402 cells
High performance liquid chromatography
