微生物酵素对免疫抑制时肠道通透性影响的实验研究

Experimental study of the effect of microbial ferment on gut barrier under immunosupression

目的:探讨免疫抑制下肠道屏障功能的变化及微生物酵素对这种变化的影响,从而为防治器官移植后肠源性感染提供依据。方法:将Wistar大鼠分成对照组(0d组)、免疫抑制组及微生物酵素治疗组,后两组又分3、5、7d3个时相点组,检测血D鄄乳酸(D鄄lactic)、二胺氧化酶(DAO)、肠道菌群微生态及肠系膜淋巴结淋巴细胞的凋亡指数等。结果:与对照组比较免疫抑制组予甲泼尼龙后各时间点血浆内D鄄乳酸均显着升高,3d及5d组升高比7d组更加明显(P<0.01,P<0.05),免疫抑制组血浆DAO较对照组均明显增高(P<0.01,P<0.05),肠道菌群较对照组无明显变化(P>0.05),肠系膜淋巴结淋巴细胞的凋亡指数在各时相点均较对照组显著升高(P<0.01);与免疫抑制组比较微生物酵素组7d时血浆D鄄乳酸含量显著降低(P<0.01),5、7d时肠道双歧杆菌、酵母菌较同时相点免疫抑制组明显升高(P<0.05,P<0.01),3、5d时肠系膜淋巴结淋巴细胞的凋亡指数明显降低(P<0.01,P<0.05),7d时两组间的凋亡指数无明显区别(P>0.05)。结论:甲泼尼龙可以造成肠屏障功能的损害,导致肠屏障的通透性升高;微生物酵素在一定程度上可以减轻甲泼尼龙的破坏作用,起到改善肠屏障,降低肠屏障通透性的保护性作用。

Objective To investigate the changes of gut barrier under immunosuppression and the effect of microbial ferment on such changes, so as to provide evidence for the prevention and therapy of enterogenic infection during transplantation. Methods Wistar rats were divided into control group(0 day), MeP(methylprednisolone) group and MiF(microbial ferment) group. Serum D-lactic, DAO, intestinal microecosystem and apoptosis of mesenteric lymphocyte were detected in days 3, 5 and 7 in eash group. Results At each time point compared with control group, D-lactic in MeP group turned to much higher(P<0.01 or P<0.05);DAO in MeP group increased significantly (P<0.01 or P<0.05);gut flora did not change much(P>0.05);the apoptosis of mesenteric lymphocyte in MeP group increased significantly(P<0.01).At each time point compared with MeP group, D-lactic in MiF group at day 7 turned to much lower(P<0.01);gut bifidobacterium(TPY) and yeast(MRS) increased significantly at days 5 and 7(P<0.05 or P<0.01);The apoptosis of mesenteric lymphocyte at days 3 and 5 was much lower(P<0.01 or P<0.05). Conclusions Methylprednisolone could damage intestinal barrier and result in its permeability increased; microbial ferment could reduce this kind of damage, improve intestinal barrier and decrease its permeability in a way.