左氧氟沙星壳聚糖缓释微球对海水浸泡创伤的初期治疗结果

Early application of levofloxacin-chitosan microspheres in the treatment of seawater immersed soft tissue wounds

目的:通过动物实验,了解左氧氟沙星壳聚糖微球对早期海水浸泡创伤的初期治疗结果。方法:建立兔软组织海水浸泡伤模型。比较对照组(n=8)和局部应用左氧氟沙星壳聚糖微球组(n=8)于海水浸泡前和浸泡结束后6、12、24、48、72h创伤局部菌落计数、血浆内毒素、局部组织和血浆TNF鄄α浓度。结果:自浸泡后6h起,对照组菌落数量显著高于微球组(P<0.01)。海水浸泡后,两组血浆内毒素水平均呈明显上升趋势;但于浸泡后6h起应用缓释微球组者,血浆内毒素水平显著低于对照组(P<0.01)。在海水浸泡后,两组TNF鄄α均呈明显上升趋势;但自浸泡后6h起,微球组局部组织TNF鄄α浓度显著低于对照组(P<0.05);12h时微球组血浆TNF鄄α浓度显著低于对照组(P<0.01)。结论:海水浸泡伤局部应用缓释制剂者,可发挥控制局部炎症反应、减轻局部伤道组织的继发损伤的作用。

Objective To evaluate the effect of early application of levofloxacin-chitosan microspheres in the treatment of seawater immersed soft tissue wounds. Methods A model of seawater immersed soft tissue wounds was established in the rabbit. In the control group(n=8) and the treatment group with local application of levofloxacin-chitosan microspheres(n=8), the number of bacteria in the wound , the serum endotoxin level, the content of tumor necrosis factor-alpha(TNF- were assayed before and 6, 12, 24, 48, 72 h after seawater immersion. Results The number of bacteria in the wound of the treatment group was significantly lower than that of the control group after 6 h of seawater immersion(P<0.01). The endotoxin level in both groups rose after seawater immersion ,the level in the control group being significantly higher than that of the treatment group(P<0.01) starting 6 h after immersion. In both groups, the TNF- content in the wound and in the serum increased after the immersion, however, the TNF- content of the wound in the treatment group was significantly lower than that in the control group starting 6 h after immersion(P<0.05), and a similar difference could be found in the serum starting 12 h after immersion(P<0.01). Conclusions Local application of levofloxacin-chitosan microspheres can reduce the inflammatory reaction of the wound, and allay the secondary injury of local tissue.