伤寒-鼠伤寒重组株Vi4072在小鼠中定居及血清学效果观察试验

Studies on the localization and serological response of the recombinant Vi 4072 in mice

将编码Vi抗原的基因克隆到减毒的鼠伤寒沙门氏菌中组建的基因重组株Vi4072,以3×10~8CFU一次口服感染Balb/C小鼠,4天后按7,14,21,28,35,42,49,56,63,70天的间隔收集分离小鼠的集合淋巴结,肝,脾.鉴别是否有本菌出现,并检测血清和小肠匀浆液中的vi抗体.结果表明,感染后49天仍可从脾中分离到该菌;70天仍可从血清和小肠匀浆液中测出vi抗体。

The Balb/c mice were fed with the recombinant strain Vi 4072 which was const ructed by genetic recombination using a aviru-tent strain of Sal.typhimurium as a receptor, and the geae coding for Vi was transformed to it. One dose was fed about 3 × 108CFU per m use. 4 days after the feeding at an interval 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70days the mouse was sacrificed.The Peyers patch. liver and spleen were collected separately for identification whether the strain fed were present.The serum andemulsion of. the small intestine were examined if there wene Vi antibodies. The results showed that the isolates were positive last for 49 days in the spleen and Vi antibodies at least 70days in the serum.