心房钠尿肽对脂多糖引起肺微血管内皮细胞损伤的治疗作用

Therapeutic effect of ANP on pulmonary microvascular endothelial cells injury induced by LPS

目的:探索心房钠尿肽(ANP)对脂多糖(LPS)引起 肺微血管内皮细胞(PMVEC)损伤的治疗作用.方法:培养大 鼠PMVECs,分为对照组、LPS(1mg/L)组和ANP治疗组(LPS+ANP),流式细胞技术检测各组细胞的周期,MTT比色 试验检测细胞活力,并测试上清液中乳酸脱氢酶(LDH)活力 和丙二醛(MDA)浓度.结果:ANP可以抑制LPS引起的S期 和G2 M期细胞比例的降低.另外,LPS作用6h后,0.01,0.1 和1μmol/L3种浓度的ANP治疗和LPS作用0、6、12和24h 后0.1μmol/L的ANP治疗,可以抑制LPS引起的A490nm值降 低和LDH活力增强(P<0.05),治疗浓度越大,治疗越早,抑制 作用越明显(P<0.05);MDA含量与LDH活力变化相似,仅 24h组无差异.结论:ANP可以减轻LPS引起的PMVECs损伤.

AIM: To investigate the therapeutic effect of atrial natriuretic peptide (ANP) on pulmonary microvascular endothelial cells (PMVECs) injury induced by lipopolysaccharide (LPS). METHODS: Cultured PMVECs were divided into control group,LPS group and ANP treatment groups (LPS+ANP).The ANP treatment group was subdivided respectively into 3 groups treated with different concentrations of ANP (0.01,0.1 and 1 μmol/L) and 4 groups treated for different time periods before adding ANP (0,6,12 and 24 h after LPS stimulation).The viability of PMVECs was examined by MTT assay and the cell cycle was analyzed by flow cytometry.The LDH activity and MDA production were measured from supernatants.RESULTS: ANP inhibited the decrease of the proportion of cells in S phase and G2-M phase induced by LPS.The A 490 nm value declined and the LDH activity and MDA production increased in LPS groups compared with those in control groups (P<0.05). But the A 490 nm value increased and the LDH activity and MDA production reduced in ANP treatment groups compared with those in LPS groups (P<0.05) in a dose-and-time dependent manner.CONCLUSION: ANP has some beneficial effects on LPS-inuced PMVECs injury.