动员自体骨髓干细胞对急性心肌缺血大鼠心功能的影响

Effect of bone marrow stem cells mobilization on heart function of acute myocardial ischemic rats

目的:探讨联合应用粒细胞集落刺激因子(G CSF) 和干细胞因子(SCF)自体动员心肌梗死大鼠的骨髓干细胞对 心功能的影响及其可能机制.方法:雄性Wistar大鼠经结扎 冠状动脉前降支制作心梗模型后分为2组.①动员组:皮下 注射rhG CSF20μg/kg,2次/d,rh SCF200μg/kg,2次/d,共 6d;②对照组:皮下注射等量生理盐水,2次/d,共6d.28d 后测定大鼠心率(HR)、左室缩-舒压差(LVEDP)、左室等容 收缩/舒张期压力上升和下降最大速率(±LVdp/dtmax),通过 伊文蓝-TTC染色方法观察心肌梗死范围的变化.结果: ①动员组左室缩-舒压差[(22.1±0.7)kPa]高于对照组 [(20.9±0.9)kPa],左室压上升/下降最大速率[(590±15) kPa/s和(440±18)kPa/s]高于对照组[(567±18)kPa/s和 (407±20)kPa/s],均具有统计学意义(P<0.05);②动员组 的缺血范围和心肌梗死的范围[(41±7)%和(43±6)

AIM: To explore the modification of heart function and the possible mechanism by mobilizing the bone marrow stem cells in acute myocardial ischemic rats.METHODS: Myocardial ischemia of left ventricle was produced in male Wistar rats by ligating the left coronary artery.The rat models were randomly divided into 2 groups.Animals in mobilization group received hypodermic injection of rhG-CSF 20 μg/kg and rh-SCF 200 μg/kg,twice per day,continuously for 6 days,and those in control group received hypodermic injection of an equivalent amount of normal saline for 6 days.After 28 days,heart function was examined and myocardial infarct size was determined by dual staining with triphenylterazolium chloride and Evan's blue. RESULTS: ① Left ventricular end diastolic pressure (LVEDP) of mobilization group [(22.1±0.7) kPa]was higher than that of control group [(20.9±0.9) kPa],and ±LVdp/dt max of mobilization group [(590±15) kPa/s and(440±18) kPa/s] was statistically higher than that of control group[(567±18) kPa/s and (407±20) kPa/s] (P<0.05);② Myocardial ischemic size and infarct size of mobilization group [(41±7)% and (43±6)%]were statistically smaller than those of control group [(53±8)% and (55±10)%] (P<0.05).CONCLUSION: G-CSF and SCF can improve the heart function of acute myocardial ischemic rats.