胎兔骨髓基质细胞与聚β-羟基丁酯(PHB)复合物修复骨缺损的实验研究

Experimental study of BMSC-PHB complex in reconstructing bone defect

目的:研究兔骨髓基质细胞(BMSC)与聚β 羟基丁 酯(PHB)复合物在成兔体内培养后成骨情况,选出最理想的 人工组织工程骨.方法:将体外培养的骨髓基质细胞种植于 PHB支架材料上形成复合物,再植入成兔体内培养,同时用单 纯PHB材料植入作对照.分别于4,8wk后照X光片,取材行 光镜和扫描电镜观察其成骨情况.结果:PHB与BMSC的复 合物在兔体内于4wk后已有骨组织形成,而对照组的缺损无 修复迹象.结论:PHB与BMSC的复合物完全可以做成理想 的组织工程人工骨修复骨缺损.

AIM: To study the bone formation of BMSC-PHB (bone marrow stromal cells-poly-β-hydroxybytyrate) complex in rabbit and to screen out the best possible human tissue-engineered bone.METHODS: BMSC was first cultured on the PHB material surface to form the BMSC-PHB complex and then the complex was implanted into rabbit mandibular bone.PHB materials without BMSC were implanted as control.The bone formation was studied respectively after 4 weeks and 8 weeks by HE staining,electron microscopy and X-ray.RESULTS: Bone formation was found on the surface of PHB after 4 weeks,while no bone formation was found in the control group.CONCLUSION: BMSC-PHB complex is an ideal tissue-engineered bone to reconstruct bone defect.