免疫抑制小鼠侵袭性肺烟曲霉菌病动物模型的建立及病理分析

Establishment and pathological analysis of animal model of invasive pulmonary aspergillosis in immunosuppressed mice

目的:建立侵袭性肺烟曲霉菌病动物模型,探讨免 疫抑制宿主曲霉菌病致病机制.方法:以甲基强的松龙为免 疫抑制剂,造成小鼠免疫抑制,对腹腔巨噬细胞和脾细胞进行 计数.双侧鼻孔滴注烟曲霉菌孢子引起侵袭性肺烟曲霉菌病, 不同时间点处死小鼠,进行组织培养及病理分析.结果:糖皮 质激素注射后小鼠免疫细胞数量急剧下降,腹腔巨噬细胞数 量明显减少[(6.33±1.76)×106vs(3.18±0.57)×106, P<0.01],脾细胞和外周血淋巴细胞数量下降(P<0.01);病 理切片可见免疫抑制小鼠感染烟曲霉菌后肺组织大量烟曲 霉菌聚积,组织坏死,形成肺脓肿.结论:成功建立了免疫抑 制小鼠侵袭性肺烟曲霉菌病动物模型,为研究免疫抑制宿主 易于感染烟曲霉菌的致病机制以及疾病的发生发展奠定基 础.

AIM: To establish an experimental model and to investigate the aspergillosis pathogenesis of invasive pulmonary aspergillosis in immunosuppressed mice.METHODS: Immunosuppressed mice were induced by prednisolone intraperitoneal injections.The quantity of peritoneal macrophages and spleen cells were counted and the Aspergillus fumigatus conidia were dropped into the nares of each mouse to cause invasive pulmonary aspergillosis.All the mice were killed at different time points and the lung tissue was cultured and histopathologically analyzed.RESULTS: The peritoneal macrophages of the mice decreased sharply after the prednisolone injection [(6.33±1.76)×10 6 vs (3.18±0.57)×10 6,P<0.01].The number of spleen cells and lymphocytes of PBMC also decreased significantly (P<0.01) .A great amount of Aspergillus fumigatus and tissue necrosis were observed histopathologically in the lungs infected with aspergillus fumigatus in immunosuppressed mice.CONCLUSION: An experimental animal model of invasive pulmonary aspergillosis is successfully established,which is useful for further studies of the aspergillosis pathogenesis and the pathological process of invasive pulmonary aspergillosis in immunosuppressed animals.