杀螟硫磷降解菌FDS-1的分离鉴定及其降解特性

Studies on isolation identification of fenitrothion degradation bacteria FDS-1 and its degradation characteristics

从长期受杀螟硫磷污染的土壤中分离到一株能以杀螟硫磷为唯一碳源生长的细菌 FDS-1,根据其生理生化分析和 16S rDNA(GenBank Accession No. AY550913)序列同源性分析,将该菌初步鉴定为伯克霍尔德氏菌属(Burkholderia sp.).该菌能在 14h 内完全降解100mg/L 的杀螟硫磷.该菌降解杀螟硫磷最适 pH 值为 7.0,最适温度为 30℃,菌株降解杀螟硫磷的速率和起始接种量呈正相关.酶的定域试验表明,该菌中有机磷水解酶为胞内酶.

A bacterium FDS-1 capable of utilizing fenitrothion as a sole carbon source was isolated from soil polluted for a long time by fenitrothion. This bacterium was identified preliminarily as Burkholderia sp. based on its physiological and biochemical analyses and 16S rDNA (GenBank Accession No. AY550913) series same source analysis. This bacterium could degrade fenitrothion of 100 mg/L entirely in 14 h. The most adaptable pH and temperature for the degradation were 7.0 and 30°C respectively. The degradation speed was related positively to initial inoculation amount. The enzyme distribution experiment showed that the organic phosphorus hydrolyzing enzyme in the bacterium was endoemzyme.