固定化优势菌种降解2,6-二叔丁基酚

Degradation of 2,6-di-tert-butylphenol by immobilized dominant bacteria

用海藻酸钙包埋固定优势降解菌(Alcaligenes sp.)降解 2,6-二叔丁基酚(2,6-2DTBP).结果表明,菌株经固定化包埋后,降解底物 2,6-DTBP的能力大大提高,在 100.0mg/L 的初始浓度下其降解率在 12d 可达到 86%.与未固定菌株相比,固定化菌株对 pH 值和温度的适应范围更宽,对底物具有更高的降解能力.对固定化菌株的降解反应过程进行动力学分析,该降解反应符合一级动力学特征,其动力学常数为 0.1519,半衰期为 4.56d.扫描电镜观察到菌种在海藻酸钙包埋载体中能良好地生长和繁殖.

The 2,6-di-tert-butylphenol (2,6-DTBP) was degraded using calcium alginate immobilized dominant bacteria (Alcaligenes sp.). After immobilizing, the bacteria capacity to degrade 2,6-DTBP was enhanced greatly. At initial concentration of 100 mg/L, the degrading rate in 12 days might reach 86%. Compared with unimmobilized bacteria, the suiting range of immobilized bacteria on pH value and temperature was much width, possessing higher degradability. Through kinetic analysis of degradation reaction process, the immobilized bacteria degradation of 2,6-DTBP tallied with one order kinetic characteristic. When 2,6-DTBP initial concentration was 100 mg/L, the kinetic constant was 0.1519, half life of 2,6-DTBP was 4.56 days. SEM images demonstrated that the bacteria immobilized in calcium alginate carrier could grow and reproduce well.