紫外线诱变原生质体选育核黄素高产菌株

SELECTION HIGH-YIELD RIBOFLAVIN PRODUCING STRAIN OF PROTOPLASTS TREATMENTED WITH UV

以产核黄素生产用菌——阿舒假囊酵母RS-89为出发菌株,用对致期生长的菌丝体,经0.5%蜗牛酶+0.5%纤维素酶作用2h可获得大量原生质体,其再生率为6.1%。对经UV诱变的原生质体再生株进行初筛、发酵复筛,从中获得了菌落大、色素深、产量高于出发菌株30%的高产稳定株——UP-91。

In this paper, factors affecting protoplast formation, the microscopic observation of the process of protopplast formation and regeneration, the distribution of Riboflavins productivity among the protoplast regenerated clonies treated with UV were investigated.Protoplasts were obtained from mycelia of Eremothecium ashbyii by using a combined lyric enzyme system containing commercial cellulase and snail digestase. The frequence of the protoplast regeneration was 6.1&, The high-yield stable strain UP-91 was obtained by UV irradiation of protoplast and screening of the fermentation of many regenerative mutants. The productivity of the mutant was as 30% high as that of starting strain.