脂多糖对人牙髓细胞增殖和碱性磷酸酶活性的影响

Proliferation and ALPase activity of human dental pulp cells in response to lipopolysaccharide in vitro

目的:了解脂多糖(LPS)对人牙髓细胞的增殖和碱性磷酸酶活性的影响。方法:Ⅰ型胶原酶消化组织块法体外培养牙髓细胞,检测细胞表面LPS受体CD14(mCD14);流式细胞术观察不同浓度LPS(0.1μg/ml、1μg/ml和10μg/ml)对牙髓细胞细胞周期的影响,测定不同浓度LPS对牙髓细胞碱性磷酸酶活性的影响,采用χ2检验以及单因素方差分析对实验数据分别进行统计学分析。结果:人牙髓细胞不表达mCD14,LPS在浓度为0.1μg/ml、1μg/ml和10μg/ml时可以抑制牙髓细胞增殖(P<0.01)及碱性磷酸酶活性(P<0.05)。结论:LPS具有抑制牙髓细胞增殖及牙髓细胞分化的作用。

PURPOSE: To investigate the effects of LPS on proliferation and ALPase activity of human dental pulp cells(HPCs). METHODS: HPCs were cultivated from connective tissue explants digested with collagenase type I.The expression of mCD14 was determined,and the effects of LPS on proliferation of HPCs at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml were observed by FCM. The effects of LPS tested on ALPase activity were examined. χ2 test and one-way ANOVA were used in statistical analysis respectively. RESULTS: The results showed that LPS receptor mCD14 was not present in HPCs. LPS inhibited proliferation of HPCs (P<0.01) and ALPase activity in HPCs (P<0.05) at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml. CONCLUSIONS: It is concluded that LPS tested inhibits proliferation of HPCs and ALPase activity of HPCs.