摘要
本文介绍一种提取和提纯隐球菌DNA的方法。将蜗牛酶消化后所得的原生质体,用SE液反复洗涤以除去夹膜多糖,并用55℃热处理裂解原生质体,使DNA的提取和提纯得到了满意的结果。每克湿菌可获得600μg以上的DNA,而且所得的DNA样品特别适合于用T_(?)法测定DNA G+以C mol%含量。
This article introduced a method to extrct and purify the DNA of Cryptococcus. Protoplasts were obtained after digested by snail enzyme. We washed them with SE liquid again and again to get rid of the capsular polysaccharides and split them with thermal treatment of 55 degrees centigrade. Then, satisfactory results of the extrction and the purification were achieved. From one wet gram of the yeast, more than 600 μg DNA can be acquired. The DNA gained is especially suited for the determination of G+C mol% content by use of the T_m method.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第1期26-28,共3页
Microbiology China
关键词
隐球菌
DNA
蜗牛酶
提取
Cryptococcus
DNA
Extraction
Snail enzyme