低剂量哌替啶抑制豚鼠吸烟所致肺内白细胞和NO水平增加(英文)

Low-dose pethidine inhibits acute cigarette smoking-induced leukocyte and nitric oxide elevation in lung in guinea pigs

目的 以支气管肺泡灌洗液(BALF)中NO水平和白细胞为指标,观察豚鼠定量吸烟后肺、气道的迟发性反应;以及很低剂量的哌替啶对这些反应的抑制作用。方法 经吸烟装置,豚鼠自主吸入 60mL香烟烟雾 (75%浓度 ) /次,共 6次,每次间隔20min。2h后检测BALF中白细胞数及其分类,以及BALF中NO2ˉ/NO3ˉ的浓度,以反映气道内NO水平。同时在药物组中观察很低剂量哌替啶对上述反应的抑制作用。结果 豚鼠定量吸烟 2h后,BALF中细胞数、中性粒细胞百分比和NO2ˉ/NO3ˉ水平显著增高;哌替啶 0. 1mg·kg-1能明显抑制上述反应。结论 多次吸烟 2h后,豚鼠肺、气道出现神经源性的迟发性炎症反应,很低剂量的哌替啶有效地抑制豚鼠吸烟产生的迟发性炎症反应。

OBJECTIVE To investigate the delayed responses after acute smoking, we observed leukocyte and nitric oxide (NO) in BAL fluid in guinea pigs; and to further investigate the inhibitory effect of low-dose pethidine against the delayed response.METHOD Cell counts were determined in BAL fluid after inhalation six volumes of cigarette smoke (60mL, 75% concentration) successively via the smoking device; and the concentration of NO_2ˉ/ NO_3ˉwas also measured to reflect the level of NO with biochemical method. The inhibitory effect against the above responses of pethidine was investigated in pethidine pre-treated group.RESULTS The total cell numbers, the percentage of neutrophils and the concentration of NO_2ˉ/ NO_3ˉ in BAL fluid were increased significantly after smoke exposure. Pethidine 0.1mg·kg -1 largely inhibited these responses.CONCLUSION A delayed neurogenic inflammation was observed in airways and parenchymal two hours after smoke exposure; and low-dose of pethidine significantly inhibit this delayed lung injury.