Q及N型Ca^(2+)通道在兔不全梗阻性不稳定膀胱中功能的初探

Studies on Q-,N-type Ca2+ channel in unstable bladder of rabbit's partial bladder outlet obstruction

目的:对Q及N型Ca2+通道在兔不全梗阻性不稳定膀胱中的功能进行研究。方法:成年同龄雄性新西兰白兔30只,其中15只为对照组(仅仅手术游离兔膀胱颈而不做结扎梗阻为对照组),15只为膀胱出口不全梗阻8周尿流动力学证实为不稳定膀胱者为实验组。模型成熟后,采用急性酶法分离及传代培养的方法获得单个膀胱平滑肌细胞。运用膀胱平滑肌M受体激动剂和Q及N型Ca2+通道阻滞剂,进行共聚焦显微镜检测其Ca2+浓度变化;了解膀胱平滑肌细胞是否存在Q及N型Ca2+通道;运用免疫组织化学方法进一步证实膀胱平滑肌细胞上是否存在Q及N型Ca2+通道及其变化。结果:共聚焦显微镜及免疫组化均证实膀胱平滑肌细胞膜存在Q及N型Ca2+通道,且不稳定膀胱平滑肌细胞膜之Q及N型Ca2+通道数量明显增多。结论:膀胱平滑肌细胞Ca2+及其通道病理性改变是出现不稳定膀胱的重要因素之一。

Objective:To study Q-,N-type Ca2+ channel in the unstable bladder of rabbit's partial bladder outlet obstruction.Methods:Thirty male New Zealand white rabbits had been divided into 2 groups randomly, the control group(15 sham operated age-matched rabbits acted as the control group, time of postoperation for 8 weeks) and the experiment group(time of model for 8 weeks, those with unstable bladder were confirmed by Urodynamics). After the model had been finished, bladder smooth muscle cells of these rabbits were isolated by collagenase digestion. Q-,N-type Ca2+ channel changes in detrusor cell between the control group and the experiment group were observed by confocal laser scanning microscope(CLSM) and immunochemical methods. Results:There were Q-,N-type Ca2+ channels in membrane of bladder smooth muscle cell with CLSM and immunochemical method. Q-,N-type Ca2+ channels significantly increased in the unstable bladder of the experiment group. Conclusion:It was an important pathogeny for the unstable bladder of partial bladder outlet obstruction that changes of pathology and pathophysiology of Ca2+ and Ca2+ channels with the unstable bladder .