血管性痴呆小鼠海马NMDA受体免疫组织化学变化特征

The features of N-methyl-D-aspartate receptor in neuron of hip pocampus of mice with vascular dementia by immunohistochemistry

目的 观测血管性痴呆小鼠海马神经元N 甲基 D 天门冬氨酸 (NMDA)受体免疫组织化学变化特征,以探讨其在血管性痴呆发病中的作用。方法 双侧颈总动脉线结,反复缺血—再灌注法制备模型,利用跳台试验和水迷宫试验观测其行为学改变,采用免疫组织化学技术观测小鼠海马神经元N 甲基 D 天门冬氨酸受体蛋白的表达变化。结果 模型组小鼠学习、记忆成绩较假手术组明显降低(P<0. 01),其海马N 甲基 D 天门冬氨酸受体蛋白表达也明显下降 (P<0. 01)。结论 血管性痴呆小鼠学习、记忆成绩下降与其海马N 甲基 D 天门冬氨酸受体蛋白低水平表达有关。

Objective The characteristics of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia (VD ) were observed for the purpose of demonstrating its function in VD pathogenesis .Methods The mice were subjected for ischemia-reperfusion repe atedly in bilateral common carotid arteries by knotting to establish the VD mode ls.The behavior abnormalities were investigated by step-down test and water m aze test,and the N-methyl-D-aspartate receptor was observed by immunohistoch emistry technique.Results The grades of learning and memory in VD mice were worse than those in sham-operation group(P<0.01),and the exp ression of N-methyl-D-aspartate receptor in VD mice was also lower than that of sham-operation group(P<0.01).Conclusion The poor grade s of learning and memory in VD mice are related to the decrease of N-methyl-D -aspartate receptor in neuron of hippocampus.