^(131)I-抗-c-erbB-2单抗对卵巢癌细胞SKOV3的抑制作用

The inhibitive effect of ^(131)I-anti-c-erbB-2-McAb on ovarian carcinoma

目的 观察1 .31 I标记针对癌蛋白p185为靶点的单克隆抗体在体外对卵巢癌细胞的抑制作用。方法 用氯胺T法1 .31 I标记抗 c erbB 2单克隆抗体 (anti c erbB 2 McAb) ,取不同浓度的1 .31 I anti c erbB 2 McAb与SKOV3细胞共同培养 ,以流式细胞术检测其对SKOV3细胞生长的抑制作用。结果 1. 31 I anti c erbB 2 McAb在体外可明显抑制SKOV3细胞 ,1 31 I nmIgG的抑制作用较弱 ,未标记的anti c erbB 2 McAb对SKOV3细胞的生长几乎没有抑制作用。结论 1. 31 I anti c erbB 2 McAb能有效抑制肿瘤细胞生长 ,有可能用于卵巢癌的免疫导向治疗。

Objective To observe the inhibitive effect of anti-oncoprotein monoclonal antibody labeled with 131I on ovarian carcinoma cell line, SKOV3. Methods Anti-c-erbB-2 monoclonal antibody (McAb) was labeled with 131I using Chloramine-T. SKOV3 cells were cultured with 131I-anti-c-erbB-2-McAb, and the inhibition was detected with flow cytometry. Results 131I-anti-c-erbB-2-McAb could inhibit the growth of SKOV3 cells, the effect of 131I-normal murine IgG (nmIgG, as control agent) was rather poor, while anti-c-erbB-2-McAb without 131I could hardly inhibit the growth of SKOV3 cells in vitro. Conclusion 131I-anti-c-erbB-2-McAb can obviously inhibit the growth of the tumor cell line and is promising to be used in the targeting radioimmunotherapy.