RP-HPLC法测定清脑宣窍方有效部位中人参皂苷Rg_1及Rb_1含量

Quantitative determination of gensenoside Rg_1 and gensenoside Rb_1 in the effective fractions of Qingnaoxuanqiao Formula by RP-HPLC

目的 建立清脑宣窍方有效部位中人参皂苷 Rg1、Rb1 HPLC 含量测定方法。方法 采用YWG C18色谱柱(250 mm×4 6 mm,10μm),检测波长:203 nm。人参皂苷 Rg1 的流动相为乙腈-0 .05 %磷酸水(21∶79),流速:1 2 mL/min,人参皂苷Rb1 的流动相为乙腈-0. 05 %磷酸水(31∶69),流速:0 9 mL/min。结果 该法人参皂苷Rg1 平均回收率为100 71 %,RSD=1 .16 %(n=5)。人参皂苷Rb1 的平均回收率为99. 32 %,RSD=2 52 %(n=5)。结论 本法操作简便、准确,可作为清脑宣窍方有效部位的质量控制方法之一。

Objective To establish a method for quantitative determination of gensenoside Rg 1 and gensenoside Rb 1 in the effective fractions of Qingnaoxuanqiao Formula by RP-HPLC. Method The determination was carried out on a YWG-C 18 column (250 mm×4.6 mm, 10 μm), and the detection wavelength was 203 nm. The mobile phase for gensenoside Rg 1 was CH 3CN-0.05%H 3PO 4 (21∶79) at a flow velocity of 1.2 mL/min, and the mobile phase for gensenoside Rb 1 was CH 3CN-0.05%H 3PO 4 (31∶69) at a flow velocity of 0.9 mL/min. Results The average recovery by this method for gensenoside Rg 1 was 100.71% (RSD = 1.16%, n=5); the average recovery by this method for gensenoside Rb 1 was 99.32%, (RSD=2.52%, n=5). Conclusion This method is simple and accurate, and can be used to control the quality of the effective fractions of Qingnaoxuanqiao Formula.