豚鼠变应性鼻炎模型鼻黏膜一氧化氮合酶免疫组织化学观察

The immunohistochemical observations of NOS in the nasal mucosa of allergic rhinitis animal model of guinea pigs

目的:了解豚鼠变应性鼻炎动物模型的鼻黏膜中诱导型NOS(iNOS)和内皮型NOS(eNOS)的表达、分布及地氯雷他定对其的影响。方法:实验豚鼠36只,随机分为模型组、治疗组和阴性对照组,经抗原致敏激发后,获取鼻黏膜组织,进行免疫组织化学染色,片将切片进行半定量图像分析。结果:eNOS的表达在治疗组和模型组均高于阴性对照组(分别P<0.05和P<0.01);治疗组和模型组比较无统计学意义。iNOS的表达在各组间的比较无统计学意义。eNOS除了iNOS的分布部位外,在杯状细胞中也有表达。结论:在豚鼠的变应性鼻炎模型中.eNOS可能比iNOS具有更重要的调节NO水平的功能;地氯雷他定对iNOS和eNOS的表达无影响。

Objective:To study the immunoreactivity and distribution of both iNOS and eNOS isoforms,and observe the effect of desloratadine on them.Method:The guinea pigs were sensitized and challenged followed by harvest of their nasal tissue for immunohistochemieal staining.The slides images were semiquantitatively analyzed and compared with the desloratadine treated group and negative control group.Result:Both iNOS and eNOS were positively stained in each group.The immunoreactivity of iNOS had no significant difference between groups(P>0.05),but eNOS had stronger immunoreactivity in the model group and the desloratadine treated group when compared with the negative control group(P<0.01 and P<0.05).In addition to the distribution area of iNOS,eNOS was also positive stained in the goblet cells.Desloratadine had no influence on iNOS and eNOS immunoreactivity.Conclusion:eNOS might play a more important role than iNOS in regulating the NO level of the nasal tissue of the guinea pigs suffered from allergic rbinitis,and desloratadine had no involvement in regulating the expression of iNOS and eNOS.