雌激素替代对双侧卵巢切除大鼠鼻黏膜上皮细胞凋亡的保护作用

Protective effect of estrogens replacement on apoptosis cells of nasal mocosas in ovariectomized rats

目的:观察并比较滴鼻和胃灌注尼尔雌醇对双侧卵巢切除术后所致大鼠鼻黏膜上皮细胞凋亡的保护作用及二者的差别。方法:将60只雌性大鼠随机分为:对照组(不切除卵巢)、A组(双侧卵巢切除)、B组(双侧卵巢切除加尼尔雌醇胃灌注)、C组(双侧卵巢切除加尼尔雌醇滴鼻),每组15只,用流式细胞仪分别检测各组动物术后15、30、60d鼻中隔黏膜阳性细胞百分率和荧光强度指数。结果:双侧卵巢切除后,大鼠鼻黏膜阳性细胞百分率增高;尼尔雌醇胃灌注后15d与对照组比较,差异有统计学意义(P<0.05),术后30d恢复正常;尼尔雌醇滴鼻后15、30d与对照组比较,差异有统计学意义(P<0.05),术后60d恢复正常。结论:尼尔雌醇胃灌注和滴鼻可通过减少凋亡细胞而保护大鼠鼻黏膜免受雌激素缺乏的损害,与胃灌注法相比,滴鼻法达到治疗效果所需时间较长。

Objective:To study the protective effect of nylestriol on apoptosis cells of atrophic nasal mucosas in ovariectomized rats and the difference between nasal drip and gastrogavage.Method:Sixty rats with atrophic rhinitis were divided into four groups at random(each with 15 rats):contrary group(Cg),ovariectomized group (Og),ovariectomized + nylestriol nasal drip group(ONNDg),and ovariectomized + nylestriol by gastrogavage (ONGg).Earlier apotosis cells of nasal mucosas taken from nasal septum were measured with flow cytometry.Result:After being ovariectomized,the number of apoptosis cells of mucosas increased.In ONGg,the number of apoptosis cells of mucosas increased at 15 d after operation (P<0.05),but it recovered at 30 d and 60 d after operation.In ONGg,the number of apoptosis cells of mucosas increased at 15 d and 30 d after operation (P<0.05),but it recovered at 60 d after operation.Conclusion:Estrogen replacement by gastrogavage and via nasal drip have effects on protecting cells of mucosas from lacking of estrogen by decreasing apoptosis cells in ovariectomized rats.It might cost more time to get therapeutic effectiveness via nasal drip than by gastrogavage.