生长激素促进老年大鼠阴茎组织中nNOS神经纤维的再生

Growth Hormone Enhances Regeneration of nNOS-containing Nerve Fibers in the Aged Rat Penis

目的:研究生长激素(GH)对老年大鼠勃起功能及阴茎组织中神经型一氧化氮合酶(nNOS)神经纤维的影 响。 方法:24只老年(18月龄)雄性SD大鼠随机均分为对照组和GH处理组。GH处理组大鼠每天后肢皮下注 射重组人GH(rhGH)200μg,对照组注射生理盐水0.2ml,共3周。于第4、8周末分别取各组1/2大鼠,颈部皮下 注射阿朴吗啡(APO)评价其勃起功能,免疫组化SP法检测大鼠阴茎组织中nNOS神经纤维的数量。 结果:4周 末,GH处理组大鼠的勃起功能和nNOS神经纤维数量与对照组相比,差异均无显著性(P>0.05)。8周末,GH处 理组大鼠仅勃起次数有上升,与对照组相比差异有显著性(P<0.05),勃起率无明显改变;而阴茎组织中nNOS神 经纤维数量,GH处理组大鼠有明显的上升,对照组大鼠无明显改变,两组相比,差异有极显著性(P<0.01)。 结 论:GH促进了老年大鼠阴茎组织中nNOS神经纤维的再生,并在一定程度上改善了老年大鼠的勃起功能。

Objective: To investigate the effects of growth hormone(GH) on the erectile function and the number of neuronal nitric oxide synthase (nNOS)-containing nerve fibers in the penis of aged rats. Methods: Twenty-four aged male SD rats (18 months) were randomized into 2 groups:GH intervention group and control group. After four and eight weeks, a half of each group were selected and tested for erectile function after apomorphine (APO) injection and then sacrificed for the detection of nNOS-containing nerve fibers in the penis by streptavidin-peroxidase conjugated method(SP method). Results: After four weeks, the erectile function and the number of nNOS-containing nerve fibers showed no significant difference between the GH intervention group and the control group(P> 0.05). After eight weeks, the erection frequency was significantly different(P< 0.05) between the two groups, while the erection rate was not. The number of nNOS-containing nerve fibers in the GH intervention group was significantly larger than that in the control group(P< 0.01). Conclusion: GH enhances the regeneration of nNOS-containing nerve fibers in the penis and improves the erectile function of the aged rat.