血管内皮生长因子-A、C在大肠癌及淋巴结转移中的表达及其意义

Expression and clinical significance of vascular endothelial growth factor A、C in colorectal carcinoma and corresponding lymph node metastases

目的探讨大肠癌及淋巴结转移中血管内皮生长因子(VEGF)A、C的表达特点及其与临床病理特征的关系。方法应用链霉抗生物素蛋白过氧化酶染色方法,检测12例正常大肠黏膜、18例大肠腺瘤、63例大肠腺癌原发灶以及19例淋巴结转移灶VEGFA、C的表达状况。结果VEGFA、C在正常大肠黏膜中无阳性表达,在大肠腺瘤有少量表达,大肠腺癌原发灶中VEGFA、C阳性表达率分别为57.14%、55.56%,明显高于正常组织和腺瘤(p<0.05);19例大肠癌原发灶和淋巴结转移灶中VEGFC高表达阳性率分别为42.11%(8/19)、73.68%(14/19),两者差异有统计学意义(P<0.05)。结论VEGFA、C的过度表达与大肠腺瘤恶变及转移有密切关系,VEGFC突出地与区域淋巴结转移明显相关。

Objective To investigate the expression of vascular endothelial growth factor A、C (VEGF-A、C)in colorectal carcinoma and its corresponding lymph node metastases and the relations with clinicopathologic characteristics.Methods The expressions of VEGF-A and VEGF-C were studied by using S-P immunohistochemistry in 12 cases of normal large intestinal mucosa,18 cases of colorectal adenoma,63 cases of colorectal primary adenocarcinoma,and 19 cases of lymph node metastases.Result There was no positive expression of VEGF-A and VEGF-C in normal large intestinal mucosa.Positive expression rate of VEGF-A and VEGF-C in colorectal primary adenocarcinoma was ~57.14% (36/63)、~55.56% (35/63) respectively,which were higher than they were in normal large intestinal mucosa and colorectal adenoma and the differences were significant ^(P< ^0.05). The high expression rate of VEGF-C in 19 cases of colorectal primary adenocarcinoma and corresponding lymph node metastases was ~42.11% (8/19)、~73.68% (14/19) respectively,the differences were significant ^(P< ^0.05). Conclusions Overexpresses of VEGF-A and VEGF-C were closely correlated with carcinomatous change of colorectal adenoma and metastasis.VEGF-C may paly an important role in region lymphatic metastasis of colorectal cancer.