嗅球组织块和细胞悬液移植对脊髓损伤的修复

Repair of spinal cord injury by transplantation of olfactory bulb tissue block or cell suspension in rat

目的探讨嗅球组织块与嗅球细胞悬液髓内移植修复脊髓损伤的可行性和疗效。方法将Wistar大鼠做成脊髓半切模型,随机分成3组,髓内分别移植嗅球组织块、嗅球细胞悬液及DMEM培养液,分期进行功能联合行为学评分(CBS)及组织学检查,评价对脊髓修复情况。结果嗅球组织块与嗅球细胞悬液髓内移植3周后各时段CBS评分明显高于DMEM组(P<0.05),组织学显示前两组有嗅鞘细胞修复的轴突再生。结论嗅球组织块与嗅球细胞悬液髓内移植修复脊髓损伤不但简化了操作而且有较好的疗效,为临床应用提供了实验依据。

Objective To investigate the effect and possibility of spinal cord injury repair by transplantation of olfactory bulb tissue block or cell suspension in rats.Methods Wistar rats with T10 spinal cord hemisection were divided into 3 groups:tissue block group,cell suspension and control group,subjected to transplantion of olfactory bulb tissue block (TB),olfactory bulb cell suspension (CS) and DMEM respectively.The functional recovery of spinal cord injury was observed with combined behavioral scores at different phases.The tissue sections were made at 2 weeks and 10 weeks postoperatively to observe the axons regeneration and the survival of olfactory ensheathing cells.Results The function of the rats treated with TB or CS was significantly improved as compared with control group (P<0.05).Histology revealed that both TB group and CS group had regeneration of axons repaired by olfactory ensheathing cells.Conclusion The method of treating spinal cord injury by transplanted TB or CS not only simplified operation but also had satisfactory curative effect.