转化生长因子3β在妊高征和正常妊娠胎盘组织中的差异表达

The different expression of transforming growth factor-3βin placentas from preeclamptic and normal pregnancies.

目的 探讨转化生长因子 3β(TGF - 3β)蛋白及mRNA在妊高征和正常妊娠胎盘组织中表达的差异。方法 取 10例妊高征和 10例年龄配对的正常妊娠的胎盘组织 ,应用Westernblot方法 ,检测妊高征和正常妊娠胎盘组织中TGF -3β蛋白的表达水平 ;应用定量PCR方法 ,检测妊高征和正常妊娠胎盘组织中TGF - 3βmRNA表达水平。结果 与正常妊娠胎盘组织TGF - 3β蛋白和mRNA表达水平相比 ,妊高征胎盘组织中的TGF - 3βmRNA表达水平增加 ,妊高征胎盘组织中的TGF - 3β蛋白表达水平也明显增加。结论 TGF - 3β在妊高征胎盘组织中表达增加 ,妊高征胎盘中TGF - 3β高表达可能与妊高征的病因有关。

Objective: To investigate the different To investigate the different expression of the transforming growth factor-3β(TGF-3β)in placentas between preeclamptic and normal pregnancies. Methods: Ten placental samples from preeclamptic pregnancies and ten from gestational age-matched normal pregnancies were obtained. The level of TGF-3β protein was valued by Western blot; the level of TGF-3β mRNA was measured by real time PCR. Results: Compared with the level of protein and mRNA of TGF-3β in placentas from normal pregnancies, the TGF-3β mRNA expression increased in placentas from preeclampsia, as well as TGF-3βprotein. Conclusion: The expression of TGF-3β increased in the placentas from preeclamptic pregnancies, and the high levels of TGF-3β in preeclamptic placentas may be relate to pathogeny of preeclampsia.