亚抑菌浓度亚胺培南体外诱导铜绿假单胞菌耐药

Subinhibitory Concentration of Imipenem Induced Resistant Isolates of Pseudomonas aeruginosa in vitro

目的 探讨铜绿假单胞菌对碳青酶烯类抗生素的耐药机制。方法 分析亚抑菌浓度亚胺培南诱导耐药的铜绿假单胞菌株体外抗菌活性、OprD2 相对含量、β 内酰胺酶活性的改变。结果 与对照组相比,诱导组亚胺培南体外抗菌活性下降,OprD2 的相对含量减少,β 内酰胺酶活性明显增加。结论 铜绿假单胞菌诱导耐药可能是OprD2的缺失和β 内酰胺酶高诱导表达共同作用的结果。

OBJECTIVE To study the mechanism of carbepenems resistance in Pseudomonas aeruginosa. METHODS To analyze the changes in antibacterial activity of imipenem in vitro, relative amounts of OprD 2 and activities of β lactamase in 10 imipenem induced isolates. RESULTS As compared with control group, the activities of β lactamase were higher, the antimicrobial activities of imipenem in vitro and relative amounts of OprD 2 were lower in those of inducing group. CONCLUSIONS The loss of OprD 2 and production of large amounts of β lactamase are responsible for resistance to carbepenems together.