铜绿假单胞菌氨基糖苷类修饰酶基因分子流行病学研究

Aminoglycosides Modifying Enzyme Gene of Pseudomonas aeruginosa: A Molecular Epidemiology Study

目的 研究铜绿假单胞菌氨基糖苷类修饰酶基因分子流行病学。方法 用 PCR方法对 35 株铜绿假单胞菌的氨基糖苷类耐药相关基因、氨基糖苷类修饰酶基因 aac(3) Ⅰ、aac(3) Ⅱ、aac(3) Ⅲ、aac(3) Ⅳ、aac(6′) Ⅰ、aac(6′) Ⅱ、aph(3′) Ⅵ、ant(3″) Ⅰ和ant(2″) Ⅰ等9种基因进行了检测与分析。结果 35株中20株检出氨基糖苷类修饰酶基因(57 1%);9种基因由高到低的检出率分别为 aac(6′) Ⅱ(34 2%)、ant(2″) Ⅰ(28 6%)、aac(3) Ⅱ(17 1%)、aac(6′) Ⅰ(17 1%)、ant(3″) Ⅰ(14 3%) 、aac(3) Ⅰ(0)、aac(3) Ⅲ(0)、aac(3) Ⅳ(0)、aph(3′) Ⅵ(0)。结论 本研究表明,湖北襄樊地区铜绿假单胞菌耐氨基糖苷类药物,主要机制为产氨基糖苷类修饰酶(57 1%),本组铜绿假单胞菌中另有15株表型为氨基糖苷类药物不敏感但 9 种基因检测为阴性,可能存在其他修饰酶基因,或者存在16S rRNA基因突变。

OBJECTIVE To investigate molecular epidemiology on aminoglycoside modifying enzyme gene of Pseudomonas aeruginosa. METHODS PCR method was used to detect and analyzed P. aeruginosa aminoglycosides resistance associated genes, aminoglycosides modifying enzyme genes aac(3) Ⅰtype,aac(3) Ⅱtype, aac(3) Ⅲ type, aac(3) Ⅳ type, aac(6′) Ⅰ type, aac(6′) Ⅱ type, aph(3′) Ⅵ type, ant(3″) Ⅰtype and ant(2″) Ⅰtype in 35 P. aeruginosa strains. RESULTS Aminoglycosides modifying enzyme genes were detected in 20 of all 35 P. aeruginosa strains (57 1%). The detected rate of the nine types of genes was ordered as aac(6′) Ⅱ(34 2%), ant(2″) Ⅰ(28 6%), aac(3) Ⅱ(17 1%), aac(6′) Ⅰ(17 1%), ant(3″) Ⅰ(14 3%), aac(3) Ⅰ(0), aac(3) Ⅲ(0), aac(3) Ⅳ(0), and aph(3′) Ⅵ(0). CONCLUSIONS The study indicated that in Xiangfan area, Hubei Province the essential mechanisms of aminoglycoside resistance in P. aeruginosa result in producing aminoglycosides modifying enzyme (57 1%).In addition, in this group 15 strains are resistant to aminoglycosides but the nine types of genes are negative, the other modifying enzyme gene is possible or 16S rRNA mutation occurred.