安必洁多酶清洗液清除细菌内毒素实验研究

Bacterial Endotoxin Elimination Using 3M EPIZYME Multi-enzyme Cleaner

目的 探讨安必洁多酶清洗液清除细菌内毒素效果的可行性。方法 将被人工污染细菌内毒素的玻璃注射器浸泡在不同浓度、时间的多酶清洗液中至规定时间,然后按 GB/T14233.2 1993 行细菌内毒素检测,并与三效热原灭活剂清除效果比较。结果 安必洁多酶清洗液 1∶200 作用 30min、1∶150 作用 10min、1∶100 作用5 min细菌内毒素检测全部阴性;与800 mg/L三效热原灭活剂作用60 min效果相当。结论 安必洁多酶清洗液具有快速清除细菌内毒素的效果。

OBJECTIVE To discuss the feasibility of bacterial endotoxin elimination using 3M EPIZYME multi enzyme cleaner. METHODS Immerge glass injector with artificial soiled endotoxin to multi enzyme solution in different concentration and time point till the stated time, then inspect it for the bacterial endotoxin according to GB/T14233.2 1993, and compare with tri effect pyrogen inactive cleaner. RESULTS All of the inspection of 3M EPIZYME multi enzyme cleaner was negative whatever 30 minute action with 1∶200 concentration, 10 minute action with 1∶150 concentration and 5 minute action with 1∶200 concentration. The effect is correspond to tri effect pyrogen inactive cleaner after 60 minute action with 800mg/L. CONCLUSIONS 3M EPIZYME multi enzyme cleaner has rapid elimination effect on the bacterial endotoxin.