摘要
目的观察刺五加叶皂甙 (ASS)预处理对供心保存效果的影响 ,并探讨其机制。方法随机将兔分为对照组 (Control组 ) ;缺血预处理组(IP组) ;ASS预处理组 (ASS组 ) ;格列苯脲加ASS预处理组(Gli+ASS组 ) ;格列苯脲组 (Gli组 )。先行药物预处理 ,再离体灌注 ,停搏 ,4℃保存180min,最后行再灌注。观察心功能 (±dp/dtmax及其恢复率 )、心肌磷酸肌酸激酶 (CK -mb)释放量、心肌ATP含量和能荷(EC)。结果①与Control组比较 ,IP组和ASS组的±dp/dtmax恢复率均增加 ,CK -mb均降低 ,停搏180min的ATP及EC均提高 (P<0.05或P<0.01) ;②Gli+ASS组和Gli组分别与Control组比较 ,±dp/dtmax恢复率 ,CK -mb ,ATP和EC均无明显差异 (P>0.05) ;③Gli+ASS组与ASS组比较 ,±dp/dtmax恢复率 ,CK -mb,停搏180min的ATP、EC差异明显(P<0.05)。结论ASS预处理供心可以发挥和缺血预处理相似的心脏保护作用。开放KATP 是ASS发挥药物预处理作用的重要机制之一。
Objective To observe the effect of Acanthopanax senticosus saponins(ASS)pretreatment on donor heart hypothermic storage and discuss cardioprotection mechanism of ASS pretreatment.Methods Rabbits were randomly divided into five groups:control group was subjected to Langendorff perfusion only;ischemic preconˉditioning group(IP group)was given2cycles of2.5min ischemia followed by5min reperfusion;ASS pretreatment group(ASS group)was given ASS pretreatment,then subjected to Langendorff perfusion;glibenclamide and ASS pretreatment group(Gli+ASS group)was given glibenclamide first,then gained ASS pretreatment and Langendorff perfusion;glibenclamide group(Gli group)was given glibenclamide first,then subjected to Langendorff perfusion.All hearts were subsequently arrested by administrating St.ThomasⅡcardioplegic solution and stored at4℃for180min.Finally,hearts of five groups were reperfused by normothermic Langendorff perfusion.Heart function paˉrameters(±dp/dt max and their recovery percentage),myocardial CK_mb leakage,myocardial content of ATP and energy charge(EC)were determined.Results①Compared with control group,±dp/dt max recovery percentage inˉcreased,CK-mb decreased,ATP and EC increased in IP group following180min hypothermic storage;②Both Gli+ASS group and Gli group were similar to control group in±dp/dt max,CK_mb,ATP and EC(P>0.05);③Gli+ASS group was differnt from ASS group in±dp/dt max,CK_mb,ATP and EC following180min hypotherˉmic storage(P<0.05).Conclusions ASS pretreatment on donor heart can exert similar cardioprotection as IP.Opening ATP sensitive potassium channel is one of the important mechanisms of pharmacological pretreatment of ASS.
出处
《中国微循环》
北大核心
2005年第1期30-33,共4页
Journal of Chinese Microcirculation
关键词
刺五加叶皂甙
预处理
供心保存
Acanthopanax senticosus saponins
Pretreatment
Heart preservation