刺五加叶皂甙预处理对供心保存的实验研究

Improvement of Donor Heart Storage by Acanthopanax Senticosus Saponins Pretreatment

目的观察刺五加叶皂甙 (ASS)预处理对供心保存效果的影响 ,并探讨其机制。方法随机将兔分为对照组 (Control组 ) ;缺血预处理组(IP组) ;ASS预处理组 (ASS组 ) ;格列苯脲加ASS预处理组(Gli+ASS组 ) ;格列苯脲组 (Gli组 )。先行药物预处理 ,再离体灌注 ,停搏 ,4℃保存180min,最后行再灌注。观察心功能 (±dp/dtmax及其恢复率 )、心肌磷酸肌酸激酶 (CK -mb)释放量、心肌ATP含量和能荷(EC)。结果①与Control组比较 ,IP组和ASS组的±dp/dtmax恢复率均增加 ,CK -mb均降低 ,停搏180min的ATP及EC均提高 (P<0.05或P<0.01) ;②Gli+ASS组和Gli组分别与Control组比较 ,±dp/dtmax恢复率 ,CK -mb ,ATP和EC均无明显差异 (P>0.05) ;③Gli+ASS组与ASS组比较 ,±dp/dtmax恢复率 ,CK -mb,停搏180min的ATP、EC差异明显(P<0.05)。结论ASS预处理供心可以发挥和缺血预处理相似的心脏保护作用。开放KATP 是ASS发挥药物预处理作用的重要机制之一。

Objective To observe the effect of Acanthopanax senticosus saponins(ASS)pretreatment on donor heart hypothermic storage and discuss cardioprotection mechanism of ASS pretreatment.Methods Rabbits were randomly divided into five groups:control group was subjected to Langendorff perfusion only;ischemic preconˉditioning group(IP group)was given2cycles of2.5min ischemia followed by5min reperfusion;ASS pretreatment group(ASS group)was given ASS pretreatment,then subjected to Langendorff perfusion;glibenclamide and ASS pretreatment group(Gli+ASS group)was given glibenclamide first,then gained ASS pretreatment and Langendorff perfusion;glibenclamide group(Gli group)was given glibenclamide first,then subjected to Langendorff perfusion.All hearts were subsequently arrested by administrating St.ThomasⅡcardioplegic solution and stored at4℃for180min.Finally,hearts of five groups were reperfused by normothermic Langendorff perfusion.Heart function paˉrameters(±dp/dt max and their recovery percentage),myocardial CK_mb leakage,myocardial content of ATP and energy charge(EC)were determined.Results①Compared with control group,±dp/dt max recovery percentage inˉcreased,CK-mb decreased,ATP and EC increased in IP group following180min hypothermic storage;②Both Gli+ASS group and Gli group were similar to control group in±dp/dt max,CK_mb,ATP and EC(P>0.05);③Gli+ASS group was differnt from ASS group in±dp/dt max,CK_mb,ATP and EC following180min hypotherˉmic storage(P<0.05).Conclusions ASS pretreatment on donor heart can exert similar cardioprotection as IP.Opening ATP sensitive potassium channel is one of the important mechanisms of pharmacological pretreatment of ASS.