气相色谱-质谱法对斑点热群立克次体——中国分离株的识别研究

ANALYSIS OF FATTY ACID COMPOSITION OF SPOTTED FEVER GROUP RICKETTSIAE ISOLATED IN CHINA BY GAS CHROMATOGRAPHY

本文报告应用气相色谱-质谱法分析了13株斑点热群立克次体(7株中国分离株和6株国际标准株)泛影葡胺密度梯度离心纯化物的全细胞脂肪酸成分。结果表明,各被检测的立克次体株所含主要脂肪酸成分百分含量有区别,所得色谱图经多元聚类法处理的7株中国分离株与国际标准株中的西伯利亚立克次体相近,而与其它国际标准株则有明显区别。

In present paper,fatty acid composition of seven Chinese isolates of SFG rickettsiae and six prototype strains of SFG rickettsiae were analyzed by GC-MS.Tested prototype strains of SFG rickettsiae were R.sibirica(strains 232 and 246),R.conorii(Simko),R.rickettsi (R),R.akari(Kaplan),R.australis(W58);Chinese isolates were An-84,Se-85,W-88 (human strain),MT-84,FT-84(D.nuttalli strain),TO-85(ova of nuttalli)and Chinese reference strain-JH-74(D.nuttalli).They were propagated in yolk sacs of embryonated hen eggs and purified by centrifugation in a 30%-36%-42% discontinuous renografin density gradient.The fatty acid composition of selected strains of SFG rickettsiae was analyzed by gas chromatography,and then comparison being carried out by single linkage on mini-computer.Identification of the strains was performed based on the results obtained from GC-MS. Results showed that the fatty acid profiles of all the isolates from China wre quantita- tively similar to that of R.sibirica and quite different from other prototype strains of SFG rickettsiae.