摘要
应用 EcoRI 和 HhaI 内切酶,对波摩那血清群钩端螺旋体各型标准株的 DNA 作限制性内切酶分析(Restriction Endonuclease Analysis 简称 REA),证明所获的各型标准株(Pomona,Mczdok,Proechimys,Tropica)的酶切图谱均不相同。与以前经显微镜凝集试验(MAT)鉴定为波摩那血清群的27个猪肾标本分离株的酶切图谱比较,所有分离株均可判定为波摩那血清型钩端螺旋体。
Restriction Endonuclease Analysis(REA)was performed on DNAs from the typestrains of the Pomona serogroup of Leptospira interrogans by using EcoRI and HhaIrestriction enzyme,and the electrophoretic patterns obtained were compared withpatterns obtained from 27 isolates from pig kidneys collected at abttoirs in Victoria,which belong to Pomona serogroup previous identified by MAT.All of the isolates wereidentified as serovar Pomona.
出处
《微生物学报》
CAS
CSCD
北大核心
1993年第5期374-377,共4页
Acta Microbiologica Sinica
